Literature DB >> 2361643

An evaluation of the polymerase chain reaction method for forensic applications.

S A Westwood1, D J Werrett.   

Abstract

This paper describes the use of polymerase chain reaction (PCR) for amplifying small amounts of DNA obtained from samples of interest to the forensic scientist. A region of the HLA DQalpha (DQa) locus was amplified in DNA prepared from the following: hair roots, liquid blood, blood-stains, semen and vaginal swabs (semen free and semen contaminated). A population study was conducted using DNA from 78 unrelated individuals. The observed distribution of HLA DQa alleles varied from that reported for an American population but obeyed the Hardy-Weinberg equilibrium. Interpretation problems associated with the PCR technique are discussed.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2361643     DOI： 10.1016/0379-0738(90)90176-y

Source DB:  PubMed          Journal:  Forensic Sci Int        ISSN： 0379-0738            Impact factor:   2.395


  8 in total

1.  Methanol for preparing hair bulbs for PCR.

Authors:  C Y Han; B K Lin; H J Lin
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

2.  The distribution of the HLA-DQ alpha alleles and genotypes in the Finnish population as determined by the use of DNA amplification and allele specific oligonucleotides.

Authors:  A Sajantila; M Ström; B Budowle; P J Tienari; C Ehnholm; L Peltonen
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

3.  Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.

Authors:  J M Jung; C T Comey; D B Baer; B Budowle
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

4.  Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay.

Authors:  C Delahunty; W Ankener; Q Deng; J Eng; D A Nickerson
Journal:  Am J Hum Genet       Date:  1996-06       Impact factor: 11.025

5.  Experience with the PCR-based HLA-DQ alpha DNA typing system in routine forensic casework.

Authors:  P M Schneider; C Rittner
Journal:  Int J Legal Med       Date:  1993       Impact factor: 2.686

Review 6.  Forensic medicine and the polymerase chain reaction technique.

Authors:  R Decorte; J J Cassiman
Journal:  J Med Genet       Date:  1993-08       Impact factor: 6.318

7.  Characterisation of HLA DQ alpha for forensic purposes. Allele and genotype frequencies in British Caucasian, Afro-Caribbean and Asian populations.

Authors:  K M Sullivan; P Gill; D Lingard; J E Lygo
Journal:  Int J Legal Med       Date:  1992       Impact factor: 2.686

8.  Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates.

Authors:  Eric M Farell; Gladys Alexandre
Journal:  BMC Res Notes       Date:  2012-05-24
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.