| Literature DB >> 19107405 |
Jong Ro Kim1, Dool-Ri Oh, Mi Hye Cha, Byoung Sik Pyo, Joon Haeng Rhee, Hyon E Choy, Won Keun Oh, Young Ran Kim.
Abstract
Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.Entities:
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Year: 2008 PMID： 19107405 DOI： 10.1007/s12275-008-0232-x
Source DB: PubMed Journal: J Microbiol ISSN： 1225-8873 Impact factor: 3.422