Literature DB >> 19100289

A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24.

Angélique Biancotto1, Beda Brichacek, Silvia S Chen, Wendy Fitzgerald, Andrea Lisco, Christophe Vanpouille, Leonid Margolis, Jean-Charles Grivel.   

Abstract

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4pg to up to 20,000pgml(-1) in a volume of 50microl and can be combined with other measurements.

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Year:  2009        PMID： 19100289      PMCID： PMC4154487          DOI： 10.1016/j.jviromet.2008.11.013

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  15 in total

1.  Protein bead array for the detection of HIV-1 antibodies from fresh plasma and dried-blood-spot specimens.

Authors:  Sylvie Faucher; Alexandre Martel; Alice Sherring; Tao Ding; Laurie Malloch; John E Kim; Michèle Bergeron; Paul Sandstrom; Francis F Mandy
Journal:  Clin Chem       Date:  2004-07       Impact factor: 8.327

2.  Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.

Authors:  Richard A Respess; Ada Cachafeiro; David Withum; Susan A Fiscus; Daniel Newman; Bernard Branson; Oliviero E Varnier; Kim Lewis; Timothy J Dondero
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

3.  Evaluation of a new fourth generation enzyme-linked immunosorbent assay, the LG HIV Ag-Ab Plus, with a combined HIV p24 antigen and anti-HIV-1/2/O screening test.

Authors:  Joon-Sup Yeom; Gyo Jun; Young Chang; Mi-Jin Sohn; Seungbum Yoo; Eunkyung Kim; Seung-Ho Ryu; Hee-Jung Kang; Young-A Kim; Sun-Young Ahn; Je-Eun Cha; Sung-Tae Youn; Jae-Won Park
Journal:  J Virol Methods       Date:  2006-08-14       Impact factor: 2.014

4.  Direct screening of clinical specimens for multiple respiratory pathogens using the Genaco Respiratory Panels 1 and 2.

Authors:  John Brunstein; Eva Thomas
Journal:  Diagn Mol Pathol       Date:  2006-09

5.  Quantification of HIV-1 p24 by a highly improved ELISA: an alternative to HIV-1 RNA based treatment monitoring in patients from Abidjan, Côte d'Ivoire.

Authors:  Andre Tehe; Chantal Maurice; Debra L Hanson; Marie Y Borget; Nadine Abiola; Matthieu Maran; Daniel Yavo; Zuzana Tomasik; Jürg Böni; Jörg Schüpbach; John N Nkengasong
Journal:  J Clin Virol       Date:  2006-09-14       Impact factor: 3.168

6.  A novel and innovative quantitative kinetic software for virological colorimetric assays.

Authors:  M Giacomini; J L McDermott; A A Giri; I Martini; F B Lillo; O E Varnier
Journal:  J Virol Methods       Date:  1998-08       Impact factor: 2.014

7.  Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma.

Authors:  J Böni; M Opravil; Z Tomasik; M Rothen; L Bisset; P J Grob; R Lüthy; J Schüpbach
Journal:  AIDS       Date:  1997-05       Impact factor: 4.177

8.  p24 Antigen detection assay modified with a booster step for diagnosis and monitoring of human immunodeficiency virus type 1 infection.

Authors:  Ruengpung Sutthent; Narintorn Gaudart; Kulkanya Chokpaibulkit; Nattaya Tanliang; Chinda Kanoksinsombath; Pongsakdi Chaisilwatana
Journal:  J Clin Microbiol       Date:  2003-03       Impact factor: 5.948

9.  Profiling antibodies to Mycobacterium tuberculosis by multiplex microbead suspension arrays for serodiagnosis of tuberculosis.

Authors:  Imran H Khan; Resmi Ravindran; JoAnn Yee; Melanie Ziman; David M Lewinsohn; Marila L Gennaro; JoAnne L Flynn; Celia W Goulding; Kathryn DeRiemer; Nickolas W Lerche; Paul A Luciw
Journal:  Clin Vaccine Immunol       Date:  2007-12-12

Review 10.  HIV-1 viral load assays for resource-limited settings.

Authors:  Susan A Fiscus; Ben Cheng; Suzanne M Crowe; Lisa Demeter; Cheryl Jennings; Veronica Miller; Richard Respess; Wendy Stevens
Journal:  PLoS Med       Date:  2006-10       Impact factor: 11.069
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  34 in total

1.  Characterization of immune responses to capsid protein p24 of human immunodeficiency virus type 1 and implications for detection.

Authors:  Shixing Tang; Jiangqin Zhao; Aifeng Wang; Ragupathy Viswanath; Harri Harma; Richard F Little; Robert Yarchoan; Susan L Stramer; Phillipe N Nyambi; Sherwin Lee; Owen Wood; Eric Y Wong; Xue Wang; Indira K Hewlett
Journal:  Clin Vaccine Immunol       Date:  2010-06-09

2.  Nanoparticle-based flow virometry for the analysis of individual virions.

Authors:  Anush Arakelyan; Wendy Fitzgerald; Leonid Margolis; Jean-Charles Grivel
Journal:  J Clin Invest       Date:  2013-08-08       Impact factor: 14.808

3.  Topical tenofovir, a microbicide effective against HIV, inhibits herpes simplex virus-2 replication.

Authors:  Graciela Andrei; Andrea Lisco; Christophe Vanpouille; Andrea Introini; Emanuela Balestra; Joost van den Oord; Tomas Cihlar; Carlo-Federico Perno; Robert Snoeck; Leonid Margolis; Jan Balzarini
Journal:  Cell Host Microbe       Date:  2011-10-20       Impact factor: 21.023

4.  Flow virometry analysis of envelope glycoprotein conformations on individual HIV virions.

Authors:  Anush Arakelyan; Wendy Fitzgerald; Deborah F King; Paul Rogers; Hannah M Cheeseman; Jean-Charles Grivel; Robin J Shattock; Leonid Margolis
Journal:  Sci Rep       Date:  2017-04-19       Impact factor: 4.379

5.  Virtual Screening of Acyclovir Derivatives as Potential Antiviral Agents: Design, Synthesis, and Biological Evaluation of New Acyclic Nucleoside ProTides.

Authors:  Marco Derudas; Christophe Vanpouille; Davide Carta; Sonia Zicari; Graciela Andrei; Robert Snoeck; Andrea Brancale; Leonid Margolis; Jan Balzarini; Christopher McGuigan
Journal:  J Med Chem       Date:  2017-09-19       Impact factor: 7.446

6.  Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine.

Authors:  Elena Zaitseva; Eugene Zaitsev; Kamran Melikov; Anush Arakelyan; Mariana Marin; Rafael Villasmil; Leonid B Margolis; Gregory B Melikyan; Leonid V Chernomordik
Journal:  Cell Host Microbe       Date:  2017-07-12       Impact factor: 21.023

7.  Histocultures (tissue explants) in human retrovirology.

Authors:  Anush Arakelyan; Wendy Fitzgerald; Jean-Charles Grivel; Christophe Vanpouille; Leonid Margolis
Journal:  Methods Mol Biol       Date:  2014

8.  Monocytes but Not Lymphocytes Carrying HIV-1 on Their Surface Transmit Infection to Human Tissue Ex Vivo.

Authors:  Victor Barreto-de-Souza; Anush Arakelyan; Sonia Zicari; Leonid Margolis; Christophe Vanpouille
Journal:  J Virol       Date:  2016-10-14       Impact factor: 5.103

9.  Detection of HIV-1 p24 antigen in patients with varying degrees of viremia using an ELISA with a photochemical signal amplification system.

Authors:  Simon Bystryak; Chitrangada Acharya
Journal:  Clin Chim Acta       Date:  2016-03-03       Impact factor: 3.786

10.  Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes.

Authors:  Mélanie Merbah; Sayali Onkar; Jean-Charles Grivel; Christophe Vanpouille; Angélique Biancotto; Lydia Bonar; Eric Sanders-Buell; Gustavo Kijak; Nelson Michael; Merlin Robb; Jerome H Kim; Sodsai Tovanabutra; Agnès-Laurence Chenine
Journal:  J Virol Methods       Date:  2016-01-22       Impact factor: 2.014
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