Literature DB >> 15635029

Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.

Richard A Respess1, Ada Cachafeiro, David Withum, Susan A Fiscus, Daniel Newman, Bernard Branson, Oliviero E Varnier, Kim Lewis, Timothy J Dondero.   

Abstract

An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of >/=30,000, with sensitivity of 46.4% at a viral load of <30,000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.

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Year:  2005        PMID： 15635029      PMCID： PMC540096          DOI： 10.1128/JCM.43.1.506-508.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  A novel and innovative quantitative kinetic software for virological colorimetric assays.

Authors:  M Giacomini; J L McDermott; A A Giri; I Martini; F B Lillo; O E Varnier
Journal:  J Virol Methods       Date:  1998-08       Impact factor: 2.014

2.  Heat-mediated immune complex dissociation and enzyme-linked immunosorbent assay signal amplification render p24 antigen detection in plasma as sensitive as HIV-1 RNA detection by polymerase chain reaction.

Authors:  J Schüpbach; M Flepp; D Pontelli; Z Tomasik; R Lüthy; J Böni
Journal:  AIDS       Date:  1996-09       Impact factor: 4.177

3.  Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma.

Authors:  J Böni; M Opravil; Z Tomasik; M Rothen; L Bisset; P J Grob; R Lüthy; J Schüpbach
Journal:  AIDS       Date:  1997-05       Impact factor: 4.177

4.  Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study.

Authors:  P Bürgisser; P Vernazza; M Flepp; J Böni; Z Tomasik; U Hummel; G Pantaleo; J Schüpbach
Journal:  J Acquir Immune Defic Syndr       Date:  2000-02-01       Impact factor: 3.731

5.  Development of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 and performance of an improved AMPLICOR HIV-1 MONITOR test with isolates of diverse subtypes.

Authors:  N L Michael; S A Herman; S Kwok; K Dreyer; J Wang; C Christopherson; J P Spadoro; K K Young; V Polonis; F E McCutchan; J Carr; J R Mascola; L L Jagodzinski; M L Robb
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

6.  Performance of a modified HIV-1 p24 antigen assay for early diagnosis of HIV-1 infection in infants and prediction of mother-to-infant transmission of HIV-1 in Dar es Salaam, Tanzania.

Authors:  E Lyamuya; U Bredberg-Rådén; A Massawe; E Urassa; G Kawo; G Msemo; T Kazimoto; A Ostborn; K Karlsson; F Mhalu; G Biberfeld
Journal:  J Acquir Immune Defic Syndr Hum Retrovirol       Date:  1996-08-01

7.  Prospective evaluation of amplification-boosted ELISA for heat-denatured p24 antigen for diagnosis and monitoring of pediatric human immunodeficiency virus type 1 infection.

Authors:  D Nadal; J Böni; C Kind; O E Varnier; F Steiner; Z Tomasik; J Schüpbach
Journal:  J Infect Dis       Date:  1999-10       Impact factor: 5.226

8.  A new quantitative HIV load assay based on plasma virion reverse transcriptase activity for the different types, groups and subtypes.

Authors:  Joséphine Braun; Jean-Christophe Plantier; Marie-France Hellot; Edouard Tuaillon; Marie Gueudin; Florence Damond; Anders Malmsten; Gary E Corrigan; François Simon
Journal:  AIDS       Date:  2003-02-14       Impact factor: 4.177

9.  Affordable diagnosis of human immunodeficiency virus infection in infants by p24 antigen detection.

Authors:  Gayle G Sherman; Gwynneth Stevens; Wendy S Stevens
Journal:  Pediatr Infect Dis J       Date:  2004-02       Impact factor: 2.129

10.  Comparison of an amplified enzyme-linked immunosorbent assay with procedures based on molecular biology for assessing human immunodeficiency virus type 1 viral load.

Authors:  P L Goldschmidt; A Devillechabrolle; Z Ait-Arkoub; J T Aubin
Journal:  Clin Diagn Lab Immunol       Date:  1998-07
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  15 in total

1.  Potential of a simplified p24 assay for early diagnosis of infant human immunodeficiency virus type 1 infection in Haiti.

Authors:  Erik George; Carole Anne Beauharnais; Emilio Brignoli; Francine Noel; Gyrlande Bois; Patricia De Matteis Rouzier; Martine Altenor; Daniel Lauture; Marlène Hosty; Sapna Mehta; Peter F Wright; Jean W Pape
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

2.  Detection and quantification of human immunodeficiency virus type 1 p24 antigen in dried whole blood and plasma on filter paper stored under various conditions.

Authors:  Chung-Chen Li; Kristy D Seidel; Robert W Coombs; Lisa M Frenkel
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

3.  Comparison of two human immunodeficiency virus (HIV) RNA surrogate assays to the standard HIV RNA assay.

Authors:  Cheryl Jennings; Susan A Fiscus; Suzanne M Crowe; Aleksandra D Danilovic; Ralph J Morack; Salvatore Scianna; Ada Cachafeiro; Donald J Brambilla; Jorg Schupbach; Wendy Stevens; Richard Respess; Oliviero E Varnier; Gary E Corrigan; J Simon Gronowitz; Michael A Ussery; James W Bremer
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

4.  Diagnostic accuracy of ultrasensitive heat-denatured HIV-1 p24 antigen in non-B subtypes in Kampala, Uganda.

Authors:  L A Spacek; F Lutwama; H M Shihab; J Summerton; M R Kamya; A Ronald; O Laeyendecker; T C Quinn; H Mayanja-Kizza
Journal:  Int J STD AIDS       Date:  2011-06       Impact factor: 1.359

5.  Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.

Authors:  Janet C Patton; Gayle G Sherman; Ashraf H Coovadia; Wendy S Stevens; Tammy M Meyers
Journal:  Clin Vaccine Immunol       Date:  2006-01

6.  Analysis of the optimal cut-point for HIV-p24 antigen testing to diagnose HIV infection in HIV-exposed children from resource-constrained settings.

Authors:  M Tamhane; B Gautney; C Shiu; N Segaren; L Jeannis; C Eustache; Y Simeon-Fadois; Y H Chen; D De; S Irivinti; P Tamma; C B Thompson; S Khamadi; G K Siberry; D Persaud
Journal:  J Clin Virol       Date:  2011-02-16       Impact factor: 3.168

7.  A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24.

Authors:  Angélique Biancotto; Beda Brichacek; Silvia S Chen; Wendy Fitzgerald; Andrea Lisco; Christophe Vanpouille; Leonid Margolis; Jean-Charles Grivel
Journal:  J Virol Methods       Date:  2009-01-06       Impact factor: 2.014

8.  Detection of HIV-1 p24 antigen in patients with varying degrees of viremia using an ELISA with a photochemical signal amplification system.

Authors:  Simon Bystryak; Chitrangada Acharya
Journal:  Clin Chim Acta       Date:  2016-03-03       Impact factor: 3.786

9.  Detection of HIV-1 p24 Gag in plasma by a nanoparticle-based bio-barcode-amplification method.

Authors:  Eun-Young Kim; Jennifer Stanton; Bette T M Korber; Kendall Krebs; Derek Bogdan; Kevin Kunstman; Samuel Wu; John P Phair; Chad A Mirkin; Steven M Wolinsky
Journal:  Nanomedicine (Lond)       Date:  2008-06       Impact factor: 5.307

Review 10.  p24 revisited: a landscape review of antigen detection for early HIV diagnosis.

Authors:  Eleanor R Gray; Robert Bain; Olivia Varsaneux; Rosanna W Peeling; Molly M Stevens; Rachel A McKendry
Journal:  AIDS       Date:  2018-09-24       Impact factor: 4.177
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