Literature DB >> 19085141

Germline transformation of Caenorhabditis elegans by injection.

Pavan Kadandale1, Indrani Chatterjee, Andrew Singson.   

Abstract

Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.

Entities:  

Mesh：

Year:  2009        PMID： 19085141      PMCID： PMC2796118          DOI： 10.1007/978-1-59745-202-1_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans gene.

Authors:  W G Kelly; S Xu; M K Montgomery; A Fire
Journal:  Genetics       Date:  1997-05       Impact factor: 4.562

2.  Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.

Authors:  V Praitis; E Casey; D Collar; J Austin
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

Review 3.  DNA transformation.

Authors:  C Mello; A Fire
Journal:  Methods Cell Biol       Date:  1995       Impact factor: 1.441

4.  Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans.

Authors:  J Kimble; J Hodgkin; T Smith; J Smith
Journal:  Nature       Date:  1982-09-30       Impact factor: 49.962

5.  Extrachromosomal DNA transformation of Caenorhabditis elegans.

Authors:  D T Stinchcomb; J E Shaw; S H Carr; D Hirsh
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

6.  The genetics of Caenorhabditis elegans.

Authors:  S Brenner
Journal:  Genetics       Date:  1974-05       Impact factor: 4.562

Review 7.  Investigating C. elegans development through mosaic analysis.

Authors:  John Yochem; Robert K Herman
Journal:  Development       Date:  2003-10       Impact factor: 6.868

8.  Integrative transformation of Caenorhabditis elegans.

Authors:  A Fire
Journal:  EMBO J       Date:  1986-10       Impact factor: 11.598

9.  Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

Authors:  C C Mello; J M Kramer; D Stinchcomb; V Ambros
Journal:  EMBO J       Date:  1991-12       Impact factor: 11.598
  9 in total
  11 in total

Review 1.  EGG molecules couple the oocyte-to-embryo transition with cell cycle progression.

Authors:  Jean M Parry; Andrew Singson
Journal:  Results Probl Cell Differ       Date:  2011

2.  The sperm surface localization of the TRP-3/SPE-41 Ca2+ -permeable channel depends on SPE-38 function in Caenorhabditis elegans.

Authors:  Gunasekaran Singaravelu; Indrani Chatterjee; Sina Rahimi; Marina K Druzhinina; Lijun Kang; X Z Shawn Xu; Andrew Singson
Journal:  Dev Biol       Date:  2012-03-09       Impact factor: 3.582

3.  CRISPR-Cas9-Guided Genome Engineering in C. elegans.

Authors:  Hyun-Min Kim; Monica P Colaiácovo
Journal:  Curr Protoc Mol Biol       Date:  2016-07-01

4.  Establishing transgenic schistosomes.

Authors:  Victoria H Mann; Sutas Suttiprapa; Gabriel Rinaldi; Paul J Brindley
Journal:  PLoS Negl Trop Dis       Date:  2011-08-30

5.  Methyl 3,4-Dihydroxybenzoate Enhances Resistance to Oxidative Stressors and Lifespan in C. elegans Partially via daf-2/daf-16.

Authors:  Xiang-Nan Mi; Li-Fang Wang; Yang Hu; Jun-Ping Pan; Yi-Rong Xin; Jia-Hui Wang; Hai-Ju Geng; Song-Hui Hu; Qin Gao; Huan-Min Luo
Journal:  Int J Mol Sci       Date:  2018-06-05       Impact factor: 5.923

6.  CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans.

Authors:  Vinci Au; Erica Li-Leger; Greta Raymant; Stephane Flibotte; George Chen; Kiana Martin; Lisa Fernando; Claudia Doell; Federico I Rosell; Su Wang; Mark L Edgley; Ann E Rougvie; Harald Hutter; Donald G Moerman
Journal:  G3 (Bethesda)       Date:  2019-01-09       Impact factor: 3.154

7.  IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans.

Authors:  Cheng-Ju Kuo; Ya-Chu Hsu; Sin-Tian Wang; Bang-Yu Liou; Serene Boon-Yuean Lim; Yi-Wei Chen; Chang-Shi Chen
Journal:  Front Immunol       Date:  2020-11-30       Impact factor: 7.561

8.  Intertissue small RNA communication mediates the acquisition and inheritance of hormesis in Caenorhabditis elegans.

Authors:  Emiko Okabe; Masaharu Uno; Saya Kishimoto; Eisuke Nishida
Journal:  Commun Biol       Date:  2021-02-16

9.  Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.

Authors:  Adam D Norris; Hyun-Min Kim; Mónica P Colaiácovo; John A Calarco
Journal:  Genetics       Date:  2015-07-30       Impact factor: 4.562

10.  Heritable genome editing in C. elegans via a CRISPR-Cas9 system.

Authors:  Ari E Friedland; Yonatan B Tzur; Kevin M Esvelt; Monica P Colaiácovo; George M Church; John A Calarco
Journal:  Nat Methods       Date:  2013-06-30       Impact factor: 28.547
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.