Manipulation of acute inflammatory lung disease.

Inflammatory lung disease to innocuous antigens or infectious pathogens is a common occurrence and in some cases, life threatening. Often, the inflammatory infiltrate that accompanies these events contributes to pathology by deleterious effects on otherwise healthy tissue and by compromising lung function by consolidating (blocking) the airspaces. A fine balance, therefore, exists between a lung immune response and immune-mediated damage, and in some the "threshold of ignorance" may be set too low. In most cases, the contributing, potentially offending, cell population or immune pathway is known, as are factors that regulate them. Why then are targeted therapeutic strategies to manipulate them not more commonplace in clinical medicine? This review highlights immune homeostasis in the lung, how and why this is lost during acute lung infection, and strategies showing promise as future immune therapeutics.

Introduction

The mucosal immune system must maintain composure in the presence of an onslaught of antigenic and potentially pathogenic material. Exposed to the outside world with, in most cases, only a single epithelial cell barrier protecting them, our mucosal surfaces have developed a sophisticated system of immune exclusion, ignorance and tolerance. The best characterized of these are described in the gastrointestinal tract. An understanding of immunity in the respiratory tract has lagged behind that of the gut, and although numerous key components have emerged, the sequence of events from initial inhalation to immune pathology in the lower respiratory tract is still unclear. Despite best efforts to maintain immune homeostasis, respiratory inflammatory disease is common and significantly life threatening. This review will highlight mechanisms that maintain lung immune homeostasis and current therapeutic efforts to contain infection-induced exaggerated acute inflammation once it occurs. The respiratory tract includes the nasopharyngeal cavity, trachea and larynx, bronchi, bronchioles, and finally the alveoli. Organized lymphoid tissue is embedded in some, but importantly not all, of these stages in the respiratory tree. Similarly, draining lymph nodes are associated with only a few of these sites. The cellular composition, requirements for activation, and expansion dynamics of respiratory tract associated lymph nodes are virtually similar to any other lymph node and will therefore not be discussed in detail here. We will focus on the regulation (or de-regulation) of immune cells embedded in the respiratory tract itself.

Respiratory Immune Compartments

Considering the total surface area of the respiratory tract constitutive, embedded organized lymphoid tissue is actually quite rare (Figure 1). Organized structured lymphoid tissue exists in the nasal cavity of rodents (nasal associated lymphoid tissue, NALT) as paired lymphoid structures at the entrance to the pharyngeal duct, but identical structures in man remain elusive (for a review, see reference Bienenstock and McDermott[1]). Organized lymphoid follicles are observed in post-mortem specimens extracted from 150 children that contain occasional germinal centers, which are associated with lymphocytes in the overlying nasal epithelium and the presence of high endothelial venules. However, in adults such lymphoid tissue is disseminated across the whole nasal mucosa,[2] and is analogous to the less well-organized diffuse lymphoid tissue (termed D-NALT) lining the nasal passages of mice.[3] In man, diffuse NALT develops after birth, likely in response to antigen, and B- and T-cell responses parallel those that occur in lymph nodes. The Waldeyer's ring comprising the nasopharyngeal (upper midline in naso-pharynx, adenoids), paired tubal (around openings of auditory tube), paired palatine (either side of the oropharynx), and lingual (under the mucosa of the posterior third of the tongue) tonsil(s) are thought of as analogous structures to NALT, but are located outside of the respiratory tract and probably also contribute to gastrointestinal immunity. Experiments with mice show that, unlike peripheral lymphoid organs, NALT develops independently of lymphotoxin-α. However, its structure and function are perturbed in lymphotoxin-α-knockout mice, possibly due to impaired expression of CXCL13, C–C chemokine ligand19 (CCL19), and CCL21, which are crucial for the recruitment and placement of lymphocytes and dendritic cells (DCs).[4]

Figure 1

Schematic representation of organized and scattered lymphoid tissue associated with the respiratory tract. Expanded diagrams show the composition of BALT and a typical alveoli lumen containing alveolar macrophages and the dendrites of sub-mucosal DCs.

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The only other organized lymphoid structure described to date located within the respiratory tract is bronchus-associated lymphoid tissue (BALT) (reviewed by Bienenstock and McDermott[1]). Whether it routinely contributes to primary immune responses or maintenance of T- and B-cell memory in the respiratory tract is not known.[5, 6] However, a recent study in mice lacking peripheral lymphoid organs suggests that BALT can initiate anti-influenza immunity and provide sufficient T cells to mediate protection against a second infection.[7] Humoral immune responses elicited by BALT are primarily mediated by immunoglobulin A (IgA) and IgG produced both locally and by BALT-derived B cells that traffic to distant mucosal sites.[8, 9] Similarly located T-cell responses have been noted. On the basis of these findings, BALT can be thought of as functionally analogous to mucosal lymphoid aggregates in the intestine. Present in up to 40% of children and adolescents (to age 20), BALT is rare in the lungs of healthy adults.[10, 11] Although originally described at the bifurcations of the bronchi, immediately beneath the epithelium,[12, 13] in the absence of antigen BALT is rare[14] and may be controlled or limited by regulatory T cells.[15] Inflammation in the lung is associated with BALT neogenesis and is described in a variety of pulmonary (Moyron-Quiroz et al[7] and references therein) and non-pulmonary[16] inflammatory conditions. Homeostatic chemokines, including CCL19 and CCL21, in mice are required for development of such inducible BALT (iBALT).[4] The extent of iBALT appears to depend on the level of inflammation in the local microenvironment, and suggests that it is developed when required. Mice lacking oxidoreductases, that protect from oxidative stress, display heightened cellularity and inflammatory cytokines and iBALT is more prevalent.[17] Whether it remains associated with larger airways and persists long after resolution of inflammation is still uncertain.

Intriguingly, mice lacking peripheral lymph nodes and spleen, but retaining iBALT, clear influenza infection (albeit slower) and survive higher doses of virus than do immune-competent mice. Such lymphotoxin-knockout mice show slower generation of influenza-specific T cells that eventually reach wild-type levels, similar to antibody isotype switching to IgG and T-cell cytokine production and effector function. This indicates that immune responses generated in iBALT although slower are protective and potentially less pathologenic.[7] This may represent a qualitative difference between local and peripherally derived immune cells or simply reflect the reduced magnitude of immunity when iBALT is the only inducing immune compartment. Regardless, in the case of lung immunity, secondary lymphoid tissues are not essential for the maintenance of immunological memory, since a pulmonary infection with influenza virus is handled equally as efficiently in their absence.[18]

In Which Compartment is Lung Inflammation Detrimental?

To date no studies have shown that organized embedded lymphoid tissue such as iBALT and NALT contribute directly to inflammatory pathology in the lung. They may initiate production of immune T and B cells that then track to less organized lung immune compartments,[7, 19, 20, 21 and 22] but their neogenesis (iBALT) or continued presence (NALT), per se, is not associated with pathology. Instead, pathological lung inflammation is attributed to those compartments that lack organized lymphoid structures, the airways and lung parenchyma. This could also be said of inflammation in the gut; Peyer's Patches and mesenteric lymph nodes may not be directly associated with pathology, whereas the lamina propria is. What the lamina propria and lung parenchyma have in common is a loose scattering of non-organized immune cells and a vast surface area of potentially non-professional antigen-presenting cells (APCs); the epithelium. The epithelium expresses constitutive MHC class I and, when inflamed, MHC class II and B7 molecules.[23, 24] It can, therefore, process and present antigen and activate T cells, but can it turn them off? We believe this non-professionalism and lack of immune-cell organization leads to immune dysregulation. During inflammation, the mediastinal lymph nodes and iBALT expand in an organized and precisely compartmentalized manner. Although low frequencies of antigen-specific cells can be observed in these sites,[25] the ratio of immune-cell subsets does not significantly change. Contraction of lung-associated lymph nodes is also well controlled; again cell proportions are retained. In lung compartments devoid of organized lymphoid tissue however, immune cells are recruited in droves by a chemotactic gradient[26, 27 and 28] from infected epithelium and/or tissue resident and alveolar macrophages. Once in the lung parenchyma or the airways, they do not form structures analogous to iBALT or lymph nodes, and it is here that pathological damage occurs, Toll-like receptors ligands dominate, and inflammatory cytokines are produced by the infiltrate in abundance. Unlike the lung-associated lymph nodes, the airways and lung parenchyma, therefore, experience dramatic shifts in their cellular composition.

This is illustrated graphically in Figure 2. The approximate cellular composition in NALT, airways, and lung is shown in homeostasis and at the peak of respiratory infections by three very different pathogens, influenza virus, the bacterium Streptococcus pneumoniae, and the fungus Cryptococcus neoformans. Obviously at other stages of the infection, slightly different cells will dominate, but only the peak of inflammation is presented for clarity. For example, natural killer cells dominate in the airways at days 3–4 of a viral infection.[29] At its peak of activity, the influenza-infected airway and lung is dominated by CD8+ and CD4+ T cells,[25, 30, 31 and 32] whereas during S. pneumoniae infection, macrophages, neutrophils, and γδT cells are more abundant.[33, 34]C. neoformans in C57BL/6 mice induces an eosinophil-dominated response in the lung and airways.[35, 36 and 37] This infiltrate in the air spaces and lung for all pathogens is dramatically different to the same sites in homeostasis that contain few lymphoid cells but a prominent macrophage population.[38, 39 and 40] Note that the NALT, despite being infected with all three pathogens, does not substantially alter the proportion of immune-cell subsets present, and the same is also true for the relatively non-infected lung draining lymph nodes.

This lack of control and excessive response is only observed in a minority, but when it occurs it is life threatening. For most of us, respiratory pathogens are cleared by non-inflammatory means, including IgA that does not fix complement well, but in dimeric form agglutinates and physically excludes antigen by a process known as immune exclusion.[41, 42] What, therefore, goes wrong in a minority? To address some of these questions, we need to understand how immune homeostasis is maintained in health in these non-organized lung compartments (reviewed by Holt[43]) and what pathways contribute to immune pathology.

Epithelial cells contribute a multitude of strategies to maintain lung immune homeostasis (for a review, see reference Holt[43]). In addition to barrier function, they secrete a variety of antimicrobial substances (surfactant protein C, mucins, and antimicrobial peptides), affect airway smooth-muscle, DC, and memory T-cell activation via nitric oxide production;[44, 45] assist in cell recruitment via production of cytokines and chemokines;[46, 47] and prolong cell survival by secreting stimulating factors such as granulocyte–macrophage colony-stimulating factor.[48, 49] Raz and co-workers[50, 51] highlight an interesting pathway critical for maintaining alveolar macrophage homeostasis, involving integrin αvβ6 that localizes these cells next to epithelial expressed transforming growth factor-β (TGF-β). This may explain why these cells are refractory to migration to the draining lymph nodes.[52] For inflammation to proceed, this inhibitory pathway must be overcome, which is mediated by a Toll-like receptor-induced conformational change of macrophages, disruption of TGF-β signaling, and reduced integrin expression. We often assume that innate immunity is inactive in the absence of antigen. However, the work of Raz et al. clearly shows that active suppression is required for homeostasis. This is also observed in mice lacking components of NADPH oxidase[53] that have heightened basal levels of airway macrophage activation due to loss of feedback inhibition.

Active suppressive mechanisms, therefore, set a “threshold of ignorance”. Those that succumb continually to inflammatory lung disease may, therefore, have dysregulated homeostatic pathways or the threshold, which antigen must exceed to induce inflammation, set too low. In the cases of TGF-β-mediated suppression of alveolar macrophages, homeostasis is overcome by cleavage of the integrin tethering it to the respiratory epithelium. Homeostasis is restored when macrophage-released matrix metalloproteinases transform latent TGF-β into its active form.[51] It may, therefore, be possible to harness these pathways artificially to dampen inflammatory lung disease with the caveat that pathogen clearance may be affected by such a global anti-inflammatory strategy.[54]

Macrophages, particularly in the airways, have long been known to have an immune-suppressive phenotype. Renewal is achieved primarily via local cell proliferation; recruitment via CCL2:CCR2 does occur, although such cells may take days to mature into the classical immune-suppressive phenotype.[55] In addition to shielding the immune system from inhaled antigens,[56] they display poor phagocytic activity[57] and tend not to migrate well to draining lymph nodes. Macrophages held in homeostasis also affect other cell types that may otherwise be proinflammatory within the respiratory tract (for a review, see reference Holt[43, 58]). DC migration to the draining lymph nodes is enhanced upon macrophage depletion,[52] and T-cell-mediated inflammatory disease ensues to antigens that would otherwise have been ignored,[59] most likely due to the usual direct suppressive influence that alveolar macrophages have on DC function.[39]

Both myeloid and plasmacytoid DCs (pDCs) are present within the lung, both increase and are recruited rapidly during inflammation, and are attracted by chemokines and cytokines produced by epithelial cells and alveolar macrophages.[43, 60, 61 and 62] Myeloid DC responses are similar to counterparts found elsewhere in the body. pDCs, however, also appear to play a tolerogenic role in the respiratory tract. They have poor APC activity[63] (but once activated enhance CD8+ T-cell responses in vivo[64] and possibly CD4+ T cells at distant sites[65]), promote inhalation tolerance,[66] and can protect from development of allergic airway disease (reviewed by de Heer et al,[66] Hammad and Lambrecht,[67] and Lambrecht[68]). During acute respiratory viral infections, pDCs perform dual functions of promoting viral clearance by secretion of type I interferon (IFN), and limit inflammation by induction of interleukin-10 (IL-10) (reviewed by Grayson and Holtzman[69]). Their role in limiting lung inflammation can be clearly seen in respiratory syncytial virus (RSV)-infected mice where pDC depletion leads to increased viral replication and enhanced immunopathology in the lungs[70, 71]. DCs encounter antigen predominantly in the lung parenchyma, although microbial sampling, via dendrite projections through the epithelial cells into the airway lumen, may induce activation, maturation, and migration, arming them to support potent T-cell responses.[72] Quite what returns these cells to homeo-stasis is unknown but may involve the level of Toll-like receptor signals and/or the influence of surface expressed inhibitory receptors such as CD200R.[73] Airway epithelial cells may also control DC activity via TGF-β analogous to alveolar macrophages,[51] although this has not been proven. Whether DCs actually transmigrate into the airspaces is still controversial.[74, 75 and 76] CCR2 assists their transit across the endothelium and CCR6 their subsequent migration into the airway.[74] The recently described CD103+ (αEβ7), CD207+, CD11blo DCs express all of the requisite chemokine receptors to draw them into the airways,[77] but their presence in this compartment has not been proven.

Therapeutic Strategies to Limit Acute Lung Inflammation

Clearly, harnessing the homeostatic pathways described above may help to resolve ongoing inflammation. However, it is equally likely that immune cell types and pathways brought into the lung with inflammatory cells during inflammation also provide a therapeutic opportunity. It would not be possible to cover all of the pathways attempted to limit lung inflammation. We will, therefore, restrict our analysis to acute infectious events (i.e., not asthma) and to targeted therapeutic strategies (i.e., not global anti-inflammatories such as corticosteroids or experiments in gene-deleted animals). Immune-mediated pathology can be manipulated at any stage of its generation. From a clinical perspective, however, after onset of identifiable symptoms would be the most beneficial. For this reason it is often resolution of inflammation that is targeted, which can include modulation of cell survival, successive waves of recruitment, and ongoing innate immunity.

Modulation of Cell Survival

It is still unclear whether immune excess in the respiratory tract stems from over-exuberant recruitment, proliferation within the airspaces, and/or accumulation in the absence of clearance of innate and adaptive immune cells. Evidence suggests that the airspaces may not support efficient T-cell proliferation[78, 79 and 80] despite memory T cells acquiring bromodeoxyuridine staining at the same rate as secondary lymphoid organs.[25, 80]

It is likely that the inflammatory cytokine and chemokine cascade that ensues upon infection[81, 82, 83, 84, 85 and 86] will prolong immune-cell survival, but at the same time enhance their recruitment, either directly or by altering vascular or epithelial permeability.[87, 88] An inflammatory environment is also associated with the highest expression of “late co-stimulatory molecules” on T cells that prevent activation-induced cell death and rely on cognate ligands expressed on APCs for signalling. OX40 (CD134) is one such late co-stimulator that is restricted to recently activated T cells, whereas the ligand (OX40L) is expressed on a number of cell types, predominantly APCs.[89] Stimulation through OX40 promotes CD4 and CD8 T-cell survival, clonal expansion,[90] inhibition of regulatory T cells,[91] and enhanced immunity to a variety of pathogens.[36, 92, 93 and 94] However, during acute influenza virus infection of mice, transient blockade of OX40 is more beneficial; alleviating illness and pathology to influenza, without compromising pathogen clearance or immunological memory.[95] A permanent absence of multiple late co-stimulators, however, can compromise immunological memory.[96, 97 and 98] Manipulation of one late co-stimulatory pathway, therefore, leaves others intact to seed the memory T-cell pool.[97] This suggests that a full compliment of late co-stimulators may actually be prolonging T-cell survival and contributing to pathology. Inflammatory cytokines, such as those abundantly expressed in the airways during infection, are known to increase OX40L on APCs.[99] Due to lack of immune organization in the airways, late co-stimulatory molecules and their ligands may be downregulated too late to avoid bystander tissue damage. The observation of OX40L on inflamed endothelium highlights the difficulty in separating the effects of immune modulators on cell survival vs. cell recruitment,[100, 101] especially when ligation of OX40L on endothelial cells induces secretion of chemokines.[101, 102] 4-1BB, like OX40, is restricted to late-effector T cells and its absence or blockade impairs CD8+ T-cell responses to influenza,[103] although the effect on associated respiratory pathology is not yet known. Care must be taken, however, since not all inducible co-stimulators result in a beneficial outcome when blocked. During lung influenza infection, ICOS blockade impairs respiratory T cells to such an extent that the virus escapes clearance.[32] This is similar to treatment of influenza-infected mice with a CTLA4-Ig fusion protein (that blocks CD28 binding to B7 molecules)[104] and CD40L-knockout mice infected with C. neoformans[105] (where macrophage antimicrobial strategies are impaired). It is likely that targeting CD27 may also produce untoward side effects, since it is expressed on resting naïve and memory T cells and is crucial for the formation CD8+ T-cell responses.[96, 106] Perhaps the defining line for therapeutic potential should be placed between those late co-stimulators that absolutely depend on T-cell receptor and constitutive CD28 signaling for their expression and those that can appear in a bystander fashion in the presence of inflammatory cytokines. The likely effect of co-stimulatory blockade and the site where manipulation may have the most effect is shown in Figure 3.

Figure 3

The location of the dominant site of action of co-stimulatory molecule blockade during acute lung infection. This model assumes that antigen specific T cells are primed in the lung-associated lymph nodes (e.g., mediastinal). As such blockade of CD28 on T cells using the B7 competitor CTLA4:Ig will affect T-cell priming in organized lymphoid tissue (a). The same is also likely to be true for ICOS that is induced rapidly after T-cell receptor and CD28 signalling. By contrast, OX40 and 4-1BB are expressed at very low levels in the lung-associated lymph nodes but upregulated in the lung parenchyma and airways, most likely due to re-recognition of antigen in situ and/or the inflammatory cytokine environment. Blockade will, therefore, impact these sites rather than the lymph nodes (b). Note that OX40 and 4-1BB blockade may also affect primed T-cell migration, as their respective ligands are present on inflamed endothelium (c). In the airways, the dominant effect will be to allow activation-induced cell death to progress; in the parenchyma, however, it may be a combination of activation-induced cell death (d) and migration.

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In addition to late co-stimulatory molecules, a number of other pathways affect the longevity of lung inflammation during acute infection. Whether apoptosis is beneficial or harmful depends on the specific infection and the dominant cell type that mediates its clearance.[107] As a general rule, apoptosis favors the host in chronic and acute intracellular bacterial and viral infections (as the process clears the pathogen), but is detrimental for extracellular bacteria.[107] For example, during infection of rats with Pneumocystis, alveolar macrophage apoptosis delays clearance of the organism that can be improved by administering caspase-9 inhibitors.[108] Similarly, apoptosis of airway epithelial cells via FAS/FAS ligand is essential to prevent dissemination of Pseudomonas aeruginosa.[109] However, during influenza infection it may be more advantageous to reduce cell survival, especially in the case of TNF producing CD8+ T cells. TNF receptor-II and very late antigen--1 synergize to protect CD8 T cells in the influenza virus infected airways from apoptosis,[110] whereas engagement of Qa-1b by CD94/NKG2A transmits a negative signal that limits immune pathology.[111] It may, therefore, be possible to resolve T-cell inflammation before bystander tissue damage occurs by blocking or enhancing these surface receptors once the viral load has reduced. This would only be useful, however, if the strategy specifically targeted a defined cell population, since apoptosis of airway epithelial cells and leukocytes may be linked to the pathology observed in those infected with highly pathogenic avian influenza.[112, 113] Furthermore, apoptosis leading to systemic lymphopenia,[114] observed during influenza infection, may assist virus propagation and survival. Other respiratory pathogens, such as cytomegalovirus and parainfluenza virus, use antiapoptotic strategies to prolong survival of the cells they infect.[115] Chlamydia blocks apoptosis by affecting the release of cytochrome c from mito-chondria.[116] The extracellular pathogens Pseudomonas cepacia and S. pneumoniae cause apoptosis of neutrophils and airway epithelial cells (Table 1),[117] respectively, to aid survival. Modulation of apoptosis is, therefore, complicated; what may benefit the host for one infection would compromise it to another.

Table 1

Summary of the impact of therapeutic intervention for intracellular and extracellular acute respiratory pathogensa

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	Intracellular pathogens		Extracellular pathogens
Intervention/treatment	Pathogen clearance	Immunopathology	Pathogen clearance	Immunopathology
Apoptosis
OX40 blockade	↔	↓	↔	↓
4-1BB blockade	↓	?a	?	?
ICOS blockade	↓	↓	?	?
CTLA-4-Ig	↓	↓	?	?
Caspase inhibitors	?	?	↑	?
Migration
Anti-MIP-2	↔	↓	?	↓
Met-RANTES	↔	↓	?	?
MCP-1	↔	↓	?	?
CCR1 antagonism	?	↓	?	?
Innate modulation
Prior infection	↔	↓	↓	↓
Nitric oxide	?	?	↑	?
Antioxidants	?	↓	?	?
Free-radical scavengers	↔	↓	?	?
NF-κB inhibition	↔	↓	↓	?
Cytokine modulation
IFN-α	↑	↓	?	?
IL-1Ra	?	?	↓	↔
Anti-IL-12	?	↓	↓	?
Anti-TNF	↔	↓	↓	?
Anti-IFN-γ	↓	↓	↓	?
Anti-IL-10	?	↓	↑	↓
TGF-β	↓	↓	↓	↓

CTLA, cytotoxic T lymphocyte antigen; IFN, interferon; Ig, immunoglobulin; IL, interleukin; IL-1Ra, IL-1-receptor antagonist; MIP, macrophage inflammatory protein; NF-κB, nuclear factor-κB; TGF, transforming growth factor; TNF, tumor-necrosis factor.

aThe effect of immune manipulation conforms to general observations for the majority of intracellular or extracellular pathogens. Specific organisms may vary. ? The outcome of this manipulation is currently unknown.

Modulation of Cell Recruitment

The recruitment and trafficking of leukocytes in response to inflammation is a tightly regulated process that can tip the balance between protection from infection and immune mediated damage in the lung. In brief, it involves slow rolling (conco-mitant to the activation of leukocytes), an increase in integrin expression and avidity to regulate the rolling arrest, adhesion strengthening as well as spreading, and intravascular crawling culminating in paracellular or trans-cellular migration.[118, 119]

Since excessive cell recruitment is a feature of many acute lung infections, targeting specific molecules involved in any of the above-mentioned events may be beneficial. Blocking monoclonal antibodies against the integrin very late antigen-4 (natalizumab)[120] and lymphocyte function-associated antigen-1 (efalizumab)[121] are currently used to treat inflammatory autoimmune disorders and Crohn's disease, but are also immunosuppressive and have not been studied in the context of acute respiratory infection.[122]

The potential benefit of blocking chemokines or their receptors using competitive blockers or antagonistic compounds is well described (reviewed by Glass et al[123] and Charo and Ransohoff[124]). Secreted chemokines bind glucosaminoglycans on endothelial cells, forming chemoattractant gradients that direct cells to inflammatory sites,[125] and are classified into constitutive homeostatic and inflammatory (requiring a pro-inflammatory stimulus such as IFN-α, TNF, or microbial products) chemokines.[126] Chemokine receptors are transmembrane G-protein-coupled molecules that trigger a signal transduction event resulting in activation and firm adhesion of the migrating cell.[127] The interactions between chemokines and their receptors are functionally redundant; many chemokines bind the same receptor and one chemokine can bind several receptors. As such it can be difficult to design reliable therapeutics that disrupt the interaction of one particular chemokine with its receptor.[128] Therapeutic administration of antibodies that block macrophage inflammatory protein-2 during influenza infection reduces neutrophil recruitment by 49% and improves lung pathology without altering viral clearance.[129] However, this strategy requires testing in co-infection models, since neutrophils are critical for clearance of most respiratory bacteria that commonly cause secondary pneumonia in the presence of influenza virus.[130, 131] RANTES (CCL5) is another potential target produced by respiratory epithelial cells during a variety of viral infections. RANTES induces CCR1-, CCR3-, and CCR5-expressing T cell, eosinophil, and monocyte recruitment to the lung.[132] During RSV infection, CCL5 is expressed by infected epithelia and resident macrophages and secreted into the airway during the first 48 h of infection. Production is then taken over by newly recruited T cells. Blocking this chemokine with a competitive inhibitor (met-RANTES)[133] during a primary RSV infection reduces lung immunopathology. However, heightened cell recruitment occurs during homologous RSV re-challenge, suggesting that manipulation during the first infection severely compromises immunological memory.[134] The same strategy has been tested in a mouse model of pneumonia virus infection. This highly lethal mouse pathogen induces a disease closely resembling severe human RSV infection in man, that is abrogated by co-administration of the antiviral agent Rivabirin and met-RANTES.[135]

In contrast to inhibition of chemokines during virus-induced lung inflammation, some infections may require their administration. P. aeruginosa lung infection causes airway neutrophil infiltration that rapidly apoptose and become toxic. Administration of recombinant monocyte chemoattractant protein-1/CCL2 recruits and activates lung macrophages that clear apoptotic neutrophils before they cause pathology.[136] IFN-inducible protein 10 (CXCL10) is critical not only for clearance of Klebsiella pneumoniae,[137] but is also produced during lung viral infection where neutralization may be beneficial.[138]

Many chemokine receptors are also increased during respiratory viral infection; CXCR3, for example, is upregulated during murine gammaherpes virus b8 infection, and its absence delays viral clearance.[139] Antagonism of CCR1 reduces mortality of pneumovirus infection of mice,[140] and absence of CCR1 also prevents the RSV-induced exacerbation of asthma.[141] Relatively little has been accomplished in this area, especially therapeutically, however, due to the paucity of reagents available for chemokine-receptor blockade.

Another method for altering lymphocyte migration is to manipulate sphingosine 1-phosphate receptors that are required for egress of lymphocytes from the thymus and peripheral lymph nodes, and impact on vascular permeability. FTY720 is a novel synthetic immunosuppressive drug that inhibits lymphocyte emigration from lymphoid organs by binding and activating sphingosine 1-phosphate receptors.[142] Sphingosine 1-phosphate is known to play a role in endotoxin-induced lung injury by affecting endothelial barrier function,[143] and is currently in trial for a variety of inflammatory disorders, including transplantation,[144] but is yet to be tested in acute lung infection.

Modulation of Innate Immunity

Modulation of lung innate immunity represents another potential therapeutic target. However, once again, few studies have tested manipulating it after the onset of symptoms during acute respiratory infection; most examine the impact of an innate immunity-associated molecule using gene-depleted animals or prior to infection. Manipulation of innate immunity may seem to be akin to “shutting the stable door after the horse has bolted,” since innate immunity is activated first and drives subsequent adaptive immune responses. However, each epithelial cell or macrophage infected sends off the next wave of an immune response. At the time of clinical presentation, therefore, both innate and adaptive immunity will be in full swing. Manipulating innate immunity at this stage will assist the resolution process, but whether inhibition or activation is required will depend on the pathogen.

Infective influences on acute respiratory infection

One way to temper innate immunity is to mature the lung microenvironment or instill probiotic microbes that would compete with the survival of pathogenic microorganisms. Oral administration of Lactobacillus casei during lung S. pneumoniae infection is protective, resulting in more rapid clearance, a shorter period of septicemia, and decreased S. pneumoniae load in the lungs. This benefit is attributed to increased neutrophils, myeloperoxidase, and IL-10 that limits lung tissue damage and is likely mediated by migration of mature APCs from the gut to the lung that are better equipped for bacterial clearance.[145] Prior infection in the lung also has a beneficial effect on some subsequent acute respiratory infections through modification or maturation of the microenvironment.[146, 147, 148, 149, 150 and 151] Prior influenza infection, for example, reduces subsequent Toll-like receptor responsiveness of alveolar macrophages for prolonged periods of time.[152] Administration of microbial products, such as CpG DNA or a modified bacterial labile toxin (LTK63), also protects against an array of subsequent respiratory pathogens,[153, 154] as do chronic or acute infections in distant sites.[155, 156 and 157] The ability of pathogen-derived proteins to modify acute respiratory infections, however, is yet to be tested therapeutically.

Resveratrol, a polyphenolic compound found in red wine, inhibits nuclear factor-κB activation, decreases mortality and pro-inflammatory cytokines (TNF, IL-1β, and IL-6) to Serratia marcescens pneumonia in rats. Though this strategy increases neutrophil numbers, they resolve more rapidly.[158] Similarly, an acidic polysaccharide compound from Cordyceps militaris, an insect-borne fungus, has anti-viral properties in a murine influenza infection model.[159] Intranasal administration of the fungal polysaccharide decreases mortality and influenza viral titers, while increasing lung pro-inflammatory cytokines. In vitro, influenza infected macrophage cell lines treated with Resveratrol display enhanced inducible nitric oxide synthase and nitric oxide (NO), suggesting that this compound may function through non-specific stimulation of alveolar macrophages in vivo.[159] Enhanced innate and adaptive immunity and reduced microbial load is also observed upon therapeutic administration of retinoic acid in mice infected with Mycobacterium tuberculosis.[160] The increased numbers of macrophages, natural killer and T cells, and increased expression of IFN-γ, TNF, inducible nitric oxide synthase, IL-10, and CXCL10 may also benefit other acute respiratory bacteria or fungi, though during viral infection it would be predicted to be detrimental. Acute respiratory infections are yet to be examined.

Manipulation of oxidative stress

A critical pathway for clearance of pathogens and infected lung epithelial cells is via NO and reactive oxygen and nitrogen species. NO contributes to host defence and mediates both pro- and anti-inflammatory effects (Reviewed in[161, 162]). As with many of the therapeutic treatments discussed, the timing and extent of modulation of NO and associated free radicals is critical. While there is no clear consensus in the literature, there are a few examples of successful intervention in this pathway. In rats with P. aeruginosa pneumonia, treatment with inhaled NO post-infection improves bacterial clearance through direct bactericidal effects, increased recruitment of neutrophils to the airways[163] or enhanced endothelial permeability.[164] During K. pneumoniae infection of rats, inhaled NO also suppresses bacterial replication and decreases lung intercellular adhesion molecule-1 expression, myeloperoxidase activity, TNF levels, and nuclear factorκB activity. Thus, NO has many paracrine effects on inflammatory cells, but also promotes bacterial clearance.[165] During viral infection, the impact of NO manipulation is less clear. Inhibition of NO during RSV infection reduces pulmonary inflammation and bystander tissue damage, but viral replication increases.[166]

Similar therapeutic strategies have been employed to reduce local concentration of reactive oxygen species and reactive nitrogen species, which are produced by infected lung epithelium and macrophages. During RSV infection of mice, administration of the antioxidant, butylated hydroxyanisole, decreases illness scores, weight loss, and lung neutrophil recruitment. Again a multi-factorial attenuation of inflammation occurs.[167] The same is observed in influenza-infected mice treated with a free-radical scavenger, manganese superoxide dismutase, within 48–96 h of infection. This enzyme has potent anti-inflammatory properties, resulting in less lung consolidation and improved arterial oxygen saturation, presumably as a result of decreased tissue damage.[168] Reduced tissue damage also occurs in mice lacking superoxide dismutase or treated therapeutically with a manganic porphyrin that scavenges reactive oxygen species.[53] Therefore, inhibition of reactive oxygen species/reactive nitrogen species and NO is beneficial for acute respiratory viral infection, but likely to be detrimental for concurrent respiratory bacteria.

Manipulation of innate immune receptor signalling

Modulation of immune-receptor signaling is in its infancy with regards to acute respiratory infection and may be limited by (a) the toxicity/safety profile of available drugs, (b) formulation challenges for in vivo delivery, (c) a lack of specificity due to shared receptor associations, and (d) problematic pharmacokinetics (sustained blockage will be detrimental for protection against infection and drugs may have to be delivered locally to prevent systemic effects). The only truly therapeutic manipulation, to date, is abrogation of airway fluid clearance during RSV infection, caused by interaction of uridine triphosphate with purinergic receptors, by post-infection administration of an active metabolite of leflunomide, A77-1726. Leflunomide restores airway fluid clearance, providing symptomatic relief and reduced lung inflammation and hypoxemia, without impairing viral replication or clearance.[169]

Although signaling molecules associated with pattern recognition and cytokine receptors are well described, little is known about the complexity of innate pathways induced by a whole pathogen, especially in the lungs.[170] Controlling the signaling pathways leading to exuberant inflammation is of major interest, but a balance needs to be struck to maintain host defence against infection. A feasible approach might be by treating patients at the peak of inflammation where immune mediators are in excess and using drugs with a short half-life. This is a promising strategy, but, to our knowledge, no drugs targeting signaling components are reported efficacious in human lung infection, although they are under development.[171] A comprehensive review of relevant inhibitors of inflammatory signaling pathways can be found elsewhere.[172]

Receptors recognizing pathogens such as Toll-like receptors or nod-like receptors, but also TNF and IL-1β, which are often involved in amplification of the inflammatory response, are potent activators of NF-κB.[173] NF-κB induction is a key factor triggering inflammation in the influenza-infected epithelium.[174] Mice treated with a cell-permeable peptide that reduces NF-κB levels, via an effect on I-kappa-B kinase beta, reduces pulmonary RSV-induced inflammation.[175] Again, this strategy may negatively affect respiratory bacteria, since intra-tracheal adenovirus delivery of a dominant NF-κB inhibitor impairs clearance of respiratory P. aeruginosa,[176] despite the reduced inflammation observed with purified bacterial products.[177, 178] Peroxisome proliferator-activated receptor-γ is a nuclear receptor involved in the stress response during lung injury and attenuates inflammatory responses by inhibiting NF-κB.[179, 180] Many steroids target peroxisome proliferator-activated receptor-γ and show encouraging results in models of lung inflammation.[172] A series of natural and synthetic ligands for these receptors have been developed and RSV-specific responses in human lung epithelial cell lines are reduced by some of these agonists.[181]

Mitogen-activated protein kinase p38 and c-Jun-N-terminal kinase pathways may also provide future suitable targets. Activation of the p38 pathway is often associated with induction of NF-κB (in Toll-like receptor responses, for example) and is thought to maintain inflammatory responses by stabilizing cytokine mRNA.[182] Inhibiting the mitogen-activated protein kinase pathway may, therefore, favor termination of inflammation. Inhibitors of p38 reduce the epithelial disruption caused by RSV and Bordetella pertussis.[183, 184] Again, inhibition of this pathway, to our knowledge, although tested in asthma,[185] has not been tested in lung infection models.

Another approach to reduce pathogen-induced immunopathology during acute infection is to target signaling molecules involved in cell migration. Downstream of chemokine receptors is the phosphatidylinositol 3-kinase, which activates protein kinase C and Rho GTPases.[186] Phosphatidylinositol 3-kinase inhibitors reduce the recruitment of neutrophils and T cells in vivo.[187, 188 and 189] Although this treatment is efficient in alleviating chronic inflammation such as asthma,[190] it is not validated for acute microbial infection. In addition, use of phosphatidylino-sitol 3-kinase inhibitors may interfere with development of the innate response to bacteria.[191]

Innate (and adaptive) cytokine manipulation

With the identification of each new cytokine, a series of papers describing their manipulation in models of acute infection has followed. All of them cannot be detailed here due to space constraint, but our discussion can be limited to those that have been tested therapeutically in acute lung infection models. The first, type-I IFN, plays such an important role in limiting viral replication that they have developed strategies to avoid it. During RNA virus lung infection IFN-α is produced predominantly by alveolar macrophages (or, to a lesser extent, pDCs) whose depletion impairs viral clearance.[192] Dosing with IFN-α and a double-stranded RNA IFN-α inducer, 4 h after SARS coronaviruas infection, reduces lung viral titers.[193] Similarly, RSV or human metapneumovirus-infected BALB/c mice, treated intranasally with recombinant IFN-α, have reduced lung viral titers and inflammatory disease as compared with untreated controls.[194] Prophylactic treatment of SARS coronavirus-infected macaques with pegylated IFN-α significantly reduces viral load and pulmonary damage; post-exposure treatment is effective, although producing intermediate results.[195] Recombinant IFN-α, therefore, appears beneficial for reducing viral replication and associated pathology when administered early after infection. The influence of concomitant bacterial infection requires examination.

Other early innate cytokines important in respiratory viral and bacterial infections include IL-1, IL-12, and TNF. Their blockade or promotion, however, is complicated by the fact that respiratory bacteria tend to require them for clearance. For example, Rhinovirus induces IL-1-receptor antagonist, IL-1Ra, from airway epithelial cells, which facilitates resolution of inflammation.[196] However, IL-1Ra enhances bacterial outgrowth in the lungs of mice with pneumococcal pneumonia, without the benefit of reducing the host response.[197] Therefore, this pathway is predicted to be good for one lung infection but bad for another. Blockade of IL-12 is another example where neutralization benefits the severity of lung viral infection,[198, 199] but impairs clearance of lung Histoplasma capsulatum[200] and Legionella pneumophila[201] infections. The list continues with neutralization of TNF, which benefits immune pathology induced by influenza and RSV infection,[81, 202] but not respiratory bacteria or fungi,[203, 204] especially if treatment is prolonged (although this will depend on the precise strategy used[205]). Webster and co-workers reported recently that absence of TNF and IL-6 is of no benefit during murine influenza H5N1 infection.[206] However, up to 50% survival was observed in some experiments and the gene-depleted animals used may harbor other developmental abnormalities. Viruses induce local production of IFN-γ by T and non-T cells in the respiratory tract, and its neutralization not only reduces local lung cellularity and systemic humoral responses to influenza virus infection in mice,[207] but may also delay viral clearance.[208] IFN-γ is also required for clearance of S. pneumoniae.[209] It would appear that the new cytokine on the block, IL-17, may also present opposing effects in viral and bacterial lung infection. Suitable reagents are yet to be developed for IL-17 neutralization, but in vivo blockade of IL-23p19 alone, or in combination with IL-23/IL-12p40 (required for Th17 development), significantly reduces Mycoplasma pneumoniae-induced IL-17 and subsequent bacterial clearance, possibly via reduced neutrophil activity.[210]K. pneumoniae clearance also depends on IL-17,[211] but the influence of IL-17 or IL-23 neutralization on respiratory viral infection is unknown.

Administration of immune suppressive cytokines has been considered for infection induced lung inflammatory disease and encountered similar problems. IL-10 neutralization increases survival of mice infected with K. pneumoniae.[212] In contrast, influenza induces indoleamine 2,3-dioxygenase and IL-10 production, which may limit lung inflammation. However, treatment with an indoleamine 2,3-dioxygenase inhibitor (that would reduce IL-10) induces a 20-fold reduction in lung S. pneumoniae load.[213] Intranasal IL-10 treatment of RSV-infected mice reduces lung nuclear factorκB DNA-binding activity, chemokine gene expression, and airway inflammation.[175] Similarly, administration of TGF-β-encoding plasmid reduces inflammation to viral and fungal lung pathogens, but, without exception, prevents their clearance.[54]

Concluding Remarks

Although a plethora of strategies have been used to modulate lung inflammation during acute infection, few are tested therapeutically after the onset of clinical symptoms, and even less are tested in models of common co-existing lung pathogens. Hundreds of immune modulators are, therefore, beneficial during influenza infection, but what of the bacteria that sometimes accompany them? Would immune therapeutics work best in combination with antibiotics? Equally, selection of immune modulators requires precision in determining exactly what the patient is infected with. In the absence of this knowledge, we may apply a beneficial strategy to one supposed infection, but create an altogether different type of problem. Several gaps remain in our knowledge of how immune homeostasis is maintained in the respiratory tract, inflammatory pathways that overcome them, and the precise effector/memory phenotype of immune cells within the airways and lung parenchyma. Controversy also still surrounds the potential of “acute” respiratory infections to persist, since detection of pathogen genome is common, but few studies have been able to demonstrate classical reactivation long after the primary infection. Should persistence exist then, depending on the nature of the persisting organism, immune modulators may cause their reactivation. The development of sensitive tools for pathogen detection, and elucidation of specific gene expression patterns in patients with acute infection,[214] mean that future use of targeted immune modulators is not impossible as long as we are able to strike a balance between immune pathology and immune defence.

Disclosure

The authors declared no conflict of interest.

Examples of cell subtypes in the NALT,[1, 144, 215 and 216] airway (by bronchoalveolar lavage) and lung parenchyma in homeostasis,[38] or during influenza virus,[25, 30, 31 and 32]S. pneumonia,[33, 34 and 217] or C. neoformans[35, 36 and 37] infection. Each sector represents an approximate percentage. Alterations in dominant cell subtypes from homeostasis is shown for the peak inflammatory infiltrate of each infection (influenza=day 7, S. pneumoniae=day 4, and C. neoformans=day 12).