OBJECTIVE: Pneumonia is a frequent cause of acute respiratory distress syndrome (ARDS), and Pseudomonas aeruginosa is a leading pathogen in nosocomial pneumonia. The management of ARDS remains a major problem, and only a limited number of options can improve the oxygenation. Inhaled nitric oxide (iNO) has been widely used, although this molecule is a free radical potentially harmful through the generation of toxic radical derivatives. The goal of our study was to assess the consequences of iNO (10 ppm) in a rat model of P. aeruginosa-induced lung injury. DESIGN: The animals were exposed for 24 h to iNO after instillation of the pathogen. Distal alveolar fluid clearance (DAFC) and epithelial and endothelial permeability were measured with a double flux of radio-labeled albumin. RESULTS: DAFC and epithelial permeability were increased in pneumonia but not influenced by iNO. In contrast, endothelial permeability was statistically significantly higher in the pneumonic animals exposed to iNO than in the pneumonic group without iNO (0.24+/-0.03 vs 0.47+/-0.1, p<0.05). This increase was not related to the production of nitrate/nitrite, nor to the increase of the inflammatory response evaluated by cytokine levels in the bronchoalveolar lavage fluid (TNF-alpha, IL-6, IL-10). The alveolar recruitment of polymorphonuclear neutrophils was comparable in the pneumonic group exposed to iNO and the pneumonic group without iNO. CONCLUSION: iNO increases the endothelial permeability in P. aeruginosa pneumonia. The mechanism is not related to the production of nitrate/nitrite or to a greater inflammatory response.
OBJECTIVE:Pneumonia is a frequent cause of acute respiratory distress syndrome (ARDS), and Pseudomonas aeruginosa is a leading pathogen in nosocomial pneumonia. The management of ARDS remains a major problem, and only a limited number of options can improve the oxygenation. Inhaled nitric oxide (iNO) has been widely used, although this molecule is a free radical potentially harmful through the generation of toxic radical derivatives. The goal of our study was to assess the consequences of iNO (10 ppm) in a rat model of P. aeruginosa-induced lung injury. DESIGN: The animals were exposed for 24 h to iNO after instillation of the pathogen. Distal alveolar fluid clearance (DAFC) and epithelial and endothelial permeability were measured with a double flux of radio-labeled albumin. RESULTS:DAFC and epithelial permeability were increased in pneumonia but not influenced by iNO. In contrast, endothelial permeability was statistically significantly higher in the pneumonic animals exposed to iNO than in the pneumonic group without iNO (0.24+/-0.03 vs 0.47+/-0.1, p<0.05). This increase was not related to the production of nitrate/nitrite, nor to the increase of the inflammatory response evaluated by cytokine levels in the bronchoalveolar lavage fluid (TNF-alpha, IL-6, IL-10). The alveolar recruitment of polymorphonuclear neutrophils was comparable in the pneumonic group exposed to iNO and the pneumonic group without iNO. CONCLUSION: iNO increases the endothelial permeability in P. aeruginosa pneumonia. The mechanism is not related to the production of nitrate/nitrite or to a greater inflammatory response.
Authors: Y Sato; K R Walley; M E Klut; D English; Y D'yachkova; J C Hogg; S F van Eeden Journal: Am J Respir Crit Care Med Date: 1999-05 Impact factor: 21.405
Authors: Robert B Cotton; Håkan W Sundell; Darryl C Zeldin; Jason D Morrow; L Jackson Roberts; Tom A Hazinski; Amy B Law; Steven Steele Journal: Pediatr Res Date: 2005-12-02 Impact factor: 3.756
Authors: B Weinberger; L Fakhrzadeh; D E Heck; J D Laskin; C R Gardner; D L Laskin Journal: Am J Respir Crit Care Med Date: 1998-09 Impact factor: 21.405
Authors: N B Viget; B P Guery; F Ader; R Nevière; S Alfandari; C Creuzy; M Roussel-Delvallez; C Foucher; C M Mason; G Beaucaire; J F Pittet Journal: Am J Physiol Lung Cell Mol Physiol Date: 2000-12 Impact factor: 5.464
Authors: Vito Fanelli; Valeria Puntorieri; Barbara Assenzio; Erica L Martin; Vincenzo Elia; Martino Bosco; Luisa Delsedime; Lorenzo Del Sorbo; Andrea Ferrari; Stefano Italiano; Alessandra Ghigo; Arthur S Slutsky; Emilio Hirsch; V Marco Ranieri Journal: Intensive Care Med Date: 2010-08-19 Impact factor: 17.440
Authors: Christine M Gross; Ruslan Rafikov; Sanjiv Kumar; Saurabh Aggarwal; P Benson Ham; Mary Louise Meadows; Mary Cherian-Shaw; Archana Kangath; Supriya Sridhar; Rudolf Lucas; Stephen M Black Journal: PLoS One Date: 2015-03-18 Impact factor: 3.240
Authors: Massimo Antonelli; Elie Azoulay; Marc Bonten; Jean Chastre; Giuseppe Citerio; Giorgio Conti; Daniel De Backer; François Lemaire; Herwig Gerlach; Johan Groeneveld; Goran Hedenstierna; Duncan Macrae; Jordi Mancebo; Salvatore M Maggiore; Alexandre Mebazaa; Philipp Metnitz; Jerôme Pugin; Jan Wernerman; Haibo Zhang Journal: Intensive Care Med Date: 2008-01-04 Impact factor: 17.440