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Literature DB >> 19066865

SERS-based plasmonic nanobiosensing in single living cells.

Jonathan P Scaffidi¹, Molly K Gregas, Victoria Seewaldt, Tuan Vo-Dinh.

Abstract

In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Year: 2008 PMID： 19066865 PMCID： PMC4022298 DOI： 10.1007/s00216-008-2521-y

Source DB: PubMed Journal: Anal Bioanal Chem ISSN： 1618-2642 Impact factor: 4.142

38 in total

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21 in total

Review 1. Single cell optical imaging and spectroscopy.

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Journal: Chem Rev Date: 2013-02-14 Impact factor: 60.622

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Authors: Tuan Vo-Dinh; Hsin-Neng Wang; Jonathan Scaffidi
Journal: J Biophotonics Date: 2010-01 Impact factor: 3.207