Literature DB >> 19066501

Obtaining eggs from Xenopus laevis females.

Abstract

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.

Entities: Species

Mesh：

Year: 2008 PMID： 19066501 PMCID： PMC3110483 DOI： 10.3791/890

Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355

Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

8 in total

1. Live-cell imaging and quantitative analysis of embryonic epithelial cells in Xenopus laevis.

Authors: Sagar D Joshi; Lance A Davidson
Journal: J Vis Exp Date: 2010-05-23 Impact factor: 1.355

2. Quantification of orofacial phenotypes in Xenopus.

Authors: Allyson E Kennedy; Amanda J Dickinson
Journal: J Vis Exp Date: 2014-11-06 Impact factor: 1.355

3. Neural Explant Cultures from Xenopus laevis.

Authors: Laura Anne Lowery; Anna E R Faris; Alina Stout; David Van Vactor
Journal: J Vis Exp Date: 2012-10-15 Impact factor: 1.355

4. Photoactivation of neurons by laser-generated local heating.

Authors: Benjamin Migliori; Massimiliano Di Ventra; William Kristan
Journal: AIP Adv Date: 2012-08-23 Impact factor: 1.548

5. Small-molecule high-throughput screening utilizing Xenopus egg extract.

Authors: Matthew R Broadus; P Renee Yew; Stephen R Hann; Ethan Lee
Journal: Methods Mol Biol Date: 2015

6. Reconstitution Of β-catenin degradation in Xenopus egg extract.

Authors: Tony W Chen; Matthew R Broadus; Stacey S Huppert; Ethan Lee
Journal: J Vis Exp Date: 2014-06-17 Impact factor: 1.355

7. Chemical Screening Using Cell-Free Xenopus Egg Extract.

Authors: Matthew R Broadus; Ethan Lee
Journal: Cold Spring Harb Protoc Date: 2018-08-01

8. Production of Xenopus tropicalis egg extracts to identify microtubule-associated RNAs.

Authors: Judith A Sharp; Mike D Blower
Journal: J Vis Exp Date: 2013-06-27 Impact factor: 1.355

8 in total