Literature DB >> 19066500

Preparation and fractionation of Xenopus laevis egg extracts.

Abstract

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Entities: Species

Mesh：

Substances：
Cell Extracts

Year: 2008 PMID： 19066500 PMCID： PMC3110484 DOI： 10.3791/891

Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355

Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

5 in total

1. Study of the DNA damage checkpoint using Xenopus egg extracts.

Authors: Jeremy Willis; Darla DeStephanis; Yogin Patel; Vrushab Gowda; Shan Yan
Journal: J Vis Exp Date: 2012-11-05 Impact factor: 1.355

2. Small-molecule high-throughput screening utilizing Xenopus egg extract.

Authors: Matthew R Broadus; P Renee Yew; Stephen R Hann; Ethan Lee
Journal: Methods Mol Biol Date: 2015

3. Reconstitution Of β-catenin degradation in Xenopus egg extract.

Authors: Tony W Chen; Matthew R Broadus; Stacey S Huppert; Ethan Lee
Journal: J Vis Exp Date: 2014-06-17 Impact factor: 1.355

4. Chemical Screening Using Cell-Free Xenopus Egg Extract.

Authors: Matthew R Broadus; Ethan Lee
Journal: Cold Spring Harb Protoc Date: 2018-08-01

5. Production of Xenopus tropicalis egg extracts to identify microtubule-associated RNAs.

Authors: Judith A Sharp; Mike D Blower
Journal: J Vis Exp Date: 2013-06-27 Impact factor: 1.355

5 in total