| Literature DB >> 19061853 |
Wassim Abdulrahman1, Muriel Uhring, Isabelle Kolb-Cheynel, Jean-Marie Garnier, Dino Moras, Natacha Rochel, Didier Busso, Arnaud Poterszman.
Abstract
We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.Entities:
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Year: 2008 PMID： 19061853 DOI： 10.1016/j.ab.2008.10.044
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365