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Literature DB >> 190592

Phosphorylation of tRNA by T4 polynucleotide kinase.

Abstract

The phosphorylation of various intact tRNA species by T4 polynucleotide kinase has been studied. The apparent Michaelis constant was on the average found to be 100 times lower than for some single-stranded DNAs previously studied. (J.R. Lillehaug and K. Kleppe, (1975) Biochemistry, 14, 1221). Conditions which result in complete phosphorylation of different tRNA species have also been established. Studies on equilibrium constants and the reversibility of the reaction revealed that the phosphorylation reaction is not a true equilibrium reaction under the conditions used in this work.

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Year: 1977 PMID： 190592 PMCID： PMC342438 DOI： 10.1093/nar/4.2.373

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

8 in total

1. End group labelling of RNA and double stranded DNA by phosphate exchange catalyzed by bacteriophage T4 induced polynucleotide kinase.

Authors: G Chaconas; J H Van de Sande; R B Church
Journal: Biochem Biophys Res Commun Date: 1975-10-06 Impact factor: 3.575

2. III. The RNA component of aminoacyl-tRNA synthetase complexes isolated from mouse liver. Absence of amino acid accepting activity.

Authors: B H Berg
Journal: Biochim Biophys Acta Date: 1975-12-04

3. Effect of salts and polyamines on T4 polynucleotide kinase.

Authors: J R Lillehaug; K Kleppe
Journal: Biochemistry Date: 1975-03-25 Impact factor: 3.162

4. Three-dimensional structure of a transfer rna in two crystal forms.

Authors: J L Sussman; S Kim
Journal: Science Date: 1976-05-28 Impact factor: 47.728

5. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors: A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal: Biochemistry Date: 1973-12-04 Impact factor: 3.162

6. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors: I M Glynn; J B Chappell
Journal: Biochem J Date: 1964-01 Impact factor: 3.857

7. Kinetics and specificity of T4 polynucleotide kinase.

Authors: J R Lillehaug; K Kleppe
Journal: Biochemistry Date: 1975-03-25 Impact factor: 3.162

8. Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.

Authors: K S Szeto; D Soll
Journal: Nucleic Acids Res Date: 1974-12 Impact factor: 16.971

8 in total

6 in total

Phosphorylation of tRNA by T4 polynucleotide kinase.

1. End group labelling of RNA and double stranded DNA by phosphate exchange catalyzed by bacteriophage T4 induced polynucleotide kinase.

2. III. The RNA component of aminoacyl-tRNA synthetase complexes isolated from mouse liver. Absence of amino acid accepting activity.

3. Effect of salts and polyamines on T4 polynucleotide kinase.

4. Three-dimensional structure of a transfer rna in two crystal forms.

5. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

6. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

7. Kinetics and specificity of T4 polynucleotide kinase.

8. Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.

1. New photoreactive tRNA derivatives for probing the peptidyl transferase center of the ribosome.

2. The 5.5 protein of phage T7 inhibits H-NS through interactions with the central oligomerization domain.

3. Structural organization of the two main rDNA size classes of Ascaris lumbricoides.

4. The structure of tRNA 5 Lys from Drosophila melanogaster.

5. Syringe method for stepwise chemical synthesis of oligonucleotides.

6. Reactions at the termini of tRNA with T4 RNA ligase.