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Literature DB >> 6322104

Structural organization of the two main rDNA size classes of Ascaris lumbricoides.

Abstract

The two main rDNA size classes in the genome of Ascaris lumbricoides consist of 8.8 kb and 8.4 kb long repeating units present in a quantitative ratio of roughly 10:1. They both contain the genes coding for 18 , 5.8S and 26S ribosomal RNAs. The length heterogeneity is due to a 450 bp long spacer region localized in the longer repeating unit which begins 870 bp upstream of the 5'-end 18S gene. A few additional microheterogeneities in base sequence occur at the 5'-end of the 26S gene. The 18S, 5.8S and 26S coding regions have been mapped on both the 8.8 kb and 8.4 kb repeating units and the localization of the 5'- and 3'-ends of the 18S and 26S genes has been performed by S1 protection. No intervening sequences are present in either coding region of the two main rDNA size classes.

Entities: Species

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Year: 1984 PMID： 6322104 PMCID： PMC318579 DOI： 10.1093/nar/12.3.1313

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

41 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa.

Authors: M A Wild; J G Gall
Journal: Cell Date: 1979-03 Impact factor: 41.582

4. Molecular aspects of chromatin elimination in Ascaris lumbricoides.

Authors: H Tobler; K D Smith; H Ursprung
Journal: Dev Biol Date: 1972-02 Impact factor: 3.582

5. Ribosomal RNA genes in germ line and somatic cells of Ascaris lumbricoides.

Authors: H Tobler; E Zulauf; O Kuhn
Journal: Dev Biol Date: 1974-12 Impact factor: 3.582

6. A simple method for DNA restriction site mapping.

Authors: H O Smith; M L Birnstiel
Journal: Nucleic Acids Res Date: 1976-09 Impact factor: 16.971

7. A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.

Authors: D M Glover; D S Hogness
Journal: Cell Date: 1977-02 Impact factor: 41.582

8. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

9. The structural organization of ribosomal DNA in Drosophila melanogaster.

Authors: P K Wellauer; I B Dawid
Journal: Cell Date: 1977-02 Impact factor: 41.582

10. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

Authors: R L White; D S Hogness
Journal: Cell Date: 1977-02 Impact factor: 41.582

14 in total

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Authors: R E Davis; A Parra; P T LoVerde; E Ribeiro; G Glorioso; S Hodgson
Journal: Proc Natl Acad Sci U S A Date: 1999-07-20 Impact factor: 11.205

2. Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to alpha-amanitin.

Authors: T M Rogalski; M Golomb; D L Riddle
Journal: Genetics Date: 1990-12 Impact factor: 4.562

3. Type I-like intervening sequences are found in the rDNA of the nematode Ascaris lumbricoides.

Authors: H Neuhaus; F Müller; A Etter; H Tobler
Journal: Nucleic Acids Res Date: 1987-10-12 Impact factor: 16.971

4. The two main rDNA size classes of Ascaris lumbricoides: comparison of transcription termination and spacer organization.

Authors: E Müller; H Neuhaus; H Tobler; F Müller
Journal: Nucleic Acids Res Date: 1992-06-25 Impact factor: 16.971

5. Supernumerary heterochromatic segments associated with the nucleolar chromosomes of Pyrgomorpha conica (Orthoptera) contain methylated rDNA sequences.

Authors: J A Suja; C Antonio; J M González-García; J S Rufas
Journal: Chromosoma Date: 1993-07 Impact factor: 4.316

6. Meiosis in holocentric chromosomes: kinetic activity is randomly restricted to the chromatid ends of sex univalents in Graphosoma italicum (Heteroptera).

Authors: J M González-García; C Antonio; J A Suja; J S Rufas
Journal: Chromosome Res Date: 1996-02 Impact factor: 5.239

10. Localization of the in vivo and in vitro transcription initiation site and comparative analysis of the flanking sequences in the two main size classes of Ascaris lumbricoides rDNA.

Authors: G Briner; E Müller; H Neuhaus; E Back; F Müller; H Tobler
Journal: Nucleic Acids Res Date: 1987-08-25 Impact factor: 16.971