Literature DB >> 19052369

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of isocitrate dehydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis.

Georgios N Hatzopoulos1, Georgia Kefala, Jochen Mueller-Dieckmann.   

Abstract

Isocitrate dehydrogenase 2 (Icd-2, Rv0066c) from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected and reduced to 3.25 A resolution in space group C2. Preliminary diffraction data analysis suggests a complex packing with at least six molecules in the asymmetric unit.

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Year:  2008        PMID： 19052369      PMCID： PMC2593698          DOI： 10.1107/S1744309108035343

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

1.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

Authors:  A A Vaguine; J Richelle; S J Wodak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

2.  Microbiology. A weak link in TB bacterium is found.

Authors:  L Helmuth
Journal:  Science       Date:  2000-08-18       Impact factor: 47.728

3.  Structure of the monomeric isocitrate dehydrogenase: evidence of a protein monomerization by a domain duplication.

Authors:  Yoshiaki Yasutake; Seiya Watanabe; Min Yao; Yasuhiro Takada; Noriyuki Fukunaga; Isao Tanaka
Journal:  Structure       Date:  2002-12       Impact factor: 5.006

4.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

5.  Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.

Authors:  J H Hurley; P E Thorsness; V Ramalingam; N H Helmers; D E Koshland; R M Stroud
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

6.  The open-access high-throughput crystallization facility at EMBL Hamburg.

Authors:  Jochen Mueller-Dieckmann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-11-23

7.  'Touchdown' PCR to circumvent spurious priming during gene amplification.

Authors:  R H Don; P T Cox; B J Wainwright; K Baker; J S Mattick
Journal:  Nucleic Acids Res       Date:  1991-07-25       Impact factor: 16.971

8.  Substrate-free structure of a monomeric NADP isocitrate dehydrogenase: an open conformation phylogenetic relationship of isocitrate dehydrogenase.

Authors:  Fumie Imabayashi; Sanjukta Aich; Lata Prasad; Louis T J Delbaere
Journal:  Proteins       Date:  2006-04-01

9.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 10.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14
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  1 in total

1.  Control of carbon flux to glutamate excretion in Klebsiella pneumoniae: the role of the indigenous plasmid and its encoded isocitrate dehydrogenase.

Authors:  Mansi El-Mansi; Francois Trappey; Ewan Clark; Malcolm Campbell
Journal:  J Ind Microbiol Biotechnol       Date:  2015-09-29       Impact factor: 3.346
  1 in total

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