Literature DB >> 1904535

A beta-galactosidase deletion mutant of Lactobacillus bulgaricus reverts to generate an active enzyme by internal DNA sequence duplication.

B Mollet1, M Delley.   

Abstract

Several spontaneous Lac- deletion derivatives of the beta-galactosidase gene of Lactobacillus bulgaricus were analyzed for their phenotypic stability. We found that one of these mutants, lac139, carrying a deletion of 30 bp within the gene, was able to revert to a Lac+ phenotype. Genetical analysis of revertants indicated that an internal region of 72 bp was duplicated immediately next to the deletion site. The region involved in the duplication event is flanked by direct repeated sequences of 13 bp in length. Both events, the deletion and the duplication, were mediated by the presence of such short direct repeats. Enzymatic studies of the purified proteins indicated identical kinetic parameters, but showed considerable instability of the revertant protein.

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Year:  1991        PMID: 1904535     DOI: 10.1007/bf00260700

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  16 in total

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5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

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7.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
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8.  Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli.

Authors:  B F Schmidt; R M Adams; C Requadt; S Power; S E Mainzer
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9.  Specific duplications fostered by a DNA structure containing adjacent inverted repeat sequences.

Authors:  W H McClain
Journal:  J Mol Biol       Date:  1988-11-05       Impact factor: 5.469

10.  Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Authors:  S Lida; R Marcoli; T A Bickle
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

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5.  Galactose utilization in Lactobacillus helveticus: isolation and characterization of the galactokinase (galK) and galactose-1-phosphate uridyl transferase (galT) genes.

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6.  ISL2, a new mobile genetic element in Lactobacillus helveticus.

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Journal:  Mol Gen Genet       Date:  1994-11-01

7.  The sensitivity and significance analysis of parameters in the model of pH regulation on lactic acid production by Lactobacillus bulgaricus.

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  7 in total

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