BACKGROUND: Condyloma acuminatum are common lesions caused by human papillomavirus (HPV). HPV is associated with many human cancers, and a vaccine now prevents infection with high-risk HPV. However, eradication of established disease is difficult, indicating that these lesions are capable of local immunosuppression. OBJECTIVE: This study examines the immunohistochemical staining characteristics of condyloma acuminatum lesions for markers of cellular immunity, including T-lymphocyte subsets, dendritic cells, and infected keratinocytes and markers of antigen presentation in condyloma tissue. METHODS: Five snap-frozen, optimal cutting temperature-embedded condyloma lesions were immunostained for T-lymphocyte markers Fox P3, CD8, CD25 and molecules involved in antigen presentation. RESULTS: Condylomas demonstrated hallmarks of immunosuppression, such as increased cellular interleukin-10 production, decreased expression of transporter associated with antigen presentation, CD40, and carbonic anhydrase IX, decreased dendritic cell counts, and increased T-regulatory cell infiltration. LIMITATIONS: This study was performed with lesions from a single center, and control tissue from the same patients was not available because of lack of patient consent. CONCLUSION: These results demonstrate that condylomas induce a local immunosuppressive environment, with deficits in antigen presentation and enhancement of immunosuppressive T-regulatory cell populations. Strategies to block this immunosuppression are required to elicit effective immune responses to HPV infection.
BACKGROUND:Condyloma acuminatum are common lesions caused by human papillomavirus (HPV). HPV is associated with many humancancers, and a vaccine now prevents infection with high-risk HPV. However, eradication of established disease is difficult, indicating that these lesions are capable of local immunosuppression. OBJECTIVE: This study examines the immunohistochemical staining characteristics of condyloma acuminatum lesions for markers of cellular immunity, including T-lymphocyte subsets, dendritic cells, and infected keratinocytes and markers of antigen presentation in condyloma tissue. METHODS: Five snap-frozen, optimal cutting temperature-embedded condyloma lesions were immunostained for T-lymphocyte markers Fox P3, CD8, CD25 and molecules involved in antigen presentation. RESULTS:Condylomas demonstrated hallmarks of immunosuppression, such as increased cellular interleukin-10 production, decreased expression of transporter associated with antigen presentation, CD40, and carbonic anhydrase IX, decreased dendritic cell counts, and increased T-regulatory cell infiltration. LIMITATIONS: This study was performed with lesions from a single center, and control tissue from the same patients was not available because of lack of patient consent. CONCLUSION: These results demonstrate that condylomas induce a local immunosuppressive environment, with deficits in antigen presentation and enhancement of immunosuppressive T-regulatory cell populations. Strategies to block this immunosuppression are required to elicit effective immune responses to HPV infection.
Authors: Jonathan M Eby; Hee-Kap Kang; Jared Klarquist; Shilpak Chatterjee; Jeffrey A Mosenson; Michael I Nishimura; Elizabeth Garrett-Mayer; B Jack Longley; Victor H Engelhard; Shikhar Mehrotra; I Caroline Le Poole Journal: Pigment Cell Melanoma Res Date: 2014-07-21 Impact factor: 4.693
Authors: Bernice Y Kwong; Scott J Roberts; Tobias Silberzahn; Renata B Filler; Jason H Neustadter; Anjela Galan; Swapna Reddy; William M Lin; Peter D Ellis; Cordelia F Langford; Adrian C Hayday; Michael Girardi Journal: J Invest Dermatol Date: 2009-11-19 Impact factor: 8.551
Authors: Jared Klarquist; Cecele J Denman; Claudia Hernandez; Derek A Wainwright; Derek J Wainwright; Faith M Strickland; Andreas Overbeck; Shikar Mehrotra; Michael I Nishimura; I Caroline Le Poole Journal: Pigment Cell Melanoma Res Date: 2010-02-19 Impact factor: 4.693