Literature DB >> 19002869

A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry.

Laurent Foiry1, Jérôme Mégret, Claudine Junien, Geneviève Gourdon.   

Abstract

Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.

Entities:  

Year:  2006        PMID： 19002869      PMCID： PMC3449417          DOI： 10.1007/s10616-006-9036-1

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  6 in total

Review 1.  Flow cytometry, an overview.

Authors:  L Scott Cram
Journal:  Methods Cell Sci       Date:  2002

2.  Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice.

Authors:  B MESSIER; C P LEBLOND
Journal:  Am J Anat       Date:  1960-05

3.  Immunohistochemical detection of proliferating cells in vivo.

Authors:  A deFazio; J A Leary; D W Hedley; M H Tattersall
Journal:  J Histochem Cytochem       Date:  1987-05       Impact factor: 2.479

4.  Preparation and stability of sixteen murine tissues and organs for flow cytometric cell cycle analysis.

Authors:  E R Burns; C B Bagwell; W G Hinson; J L Pipkin; J L Hudson
Journal:  Cytometry       Date:  1983-09

5.  Isolation of mouse liver cells: perfusion technique and metabolic evaluation.

Authors:  S Edström; L Ekman; M Ternell; K Lundholm
Journal:  Eur Surg Res       Date:  1983       Impact factor: 1.745

6.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

Authors:  A Krishan
Journal:  J Cell Biol       Date:  1975-07       Impact factor: 10.539
  6 in total
  3 in total

1.  A rapid and optimization-free procedure allows the in vivo detection of subtle cell cycle and ploidy alterations in tissues by flow cytometry.

Authors:  Christina Heinlein; Wolfgang Deppert; Antony W Braithwaite; Daniel Speidel
Journal:  Cell Cycle       Date:  2010-09-01       Impact factor: 4.534

2.  Tissue- and age-specific DNA replication patterns at the CTG/CAG-expanded human myotonic dystrophy type 1 locus.

Authors:  John D Cleary; Stéphanie Tomé; Arturo López Castel; Gagan B Panigrahi; Laurent Foiry; Katharine A Hagerman; Hana Sroka; David Chitayat; Geneviève Gourdon; Christopher E Pearson
Journal:  Nat Struct Mol Biol       Date:  2010-08-15       Impact factor: 15.369

3.  Viability of Escherichia coli ATCC 8739 in Nutrient Broth, Luria-Bertani Broth and Brain Heart Infusion over 11 Weeks.

Authors:  Samuel Xin Zher Low; Zhen Qin Aw; Bryan Zong Lin Loo; Kun Cheng Lee; Jack Si Hao Oon; Chin How Lee; Maurice Han Tong Ling
Journal:  Electron Physician       Date:  2013-02-01
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.