Literature DB >> 19002828

Metabolism of amyloid precursor protein in COS cells transfected with a beta-secretase candidate.

H Koike1, Z Kouchi, T Kinouchi, T Maeda, H Sorimachi, T C Saido, K Maruyama, A Okuyama, K Suzuki, S Ishiura.   

Abstract

Thimet oligopeptidase (TOP) is a thiol- andmetallo-dependent peptidase and has been shown to beone of the beta-secretase candidates. TOPexpressed in COS cells cleaved amyloid precursorprotein (APP) at the beta-secretase site, and wefound a proteolytic product of APP called secretedform of APP by beta-secretase (sAPPbeta) in theconditioned media. Here we demonstrate thatsAPPbeta was increased in conditioned media whenTOP was coexpressed in COS cells with APP and treatedwith an ADAM inhibitor SI-27. In addition, althoughTOP expressed in COS cell was localized at nuclei orGolgi apparatus, it exclusively colocalized at Golgiapparatus when APP was coexpressed with TOP.

Entities:  

Year:  2000        PMID： 19002828      PMCID： PMC3466702          DOI： 10.1023/A:1008119512341

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  21 in total

1.  Evidence that beta-amyloid protein in Alzheimer's disease is not derived by normal processing.

Authors:  S S Sisodia; E H Koo; K Beyreuther; A Unterbeck; D L Price
Journal:  Science       Date:  1990-04-27       Impact factor: 47.728

2.  The deletion of the C-terminal tail and addition of an endoplasmic reticulum targeting signal to Alzheimer's amyloid precursor protein change its localization, secretion, and intracellular proteolysis.

Authors:  Z Kouchi; T Kinouchi; H Sorimachi; S Ishiura; K Suzuki
Journal:  Eur J Biochem       Date:  1998-12-01

3.  Electroporation for the efficient transfection of mammalian cells with DNA.

Authors:  G Chu; H Hayakawa; P Berg
Journal:  Nucleic Acids Res       Date:  1987-02-11       Impact factor: 16.971

4.  Thimet oligopeptidase cleaves the full-length Alzheimer amyloid precursor protein at a beta-secretase cleavage site in COS cells.

Authors:  H Koike; H Seki; Z Kouchi; M Ito; T Kinouchi; S Tomioka; H Sorimachi; T C Saido; K Maruyama; K Suzuki; S Ishiura
Journal:  J Biochem       Date:  1999-07       Impact factor: 3.387

5.  Cleavage of amyloid beta peptide during constitutive processing of its precursor.

Authors:  F S Esch; P S Keim; E C Beattie; R W Blacher; A R Culwell; T Oltersdorf; D McClure; P J Ward
Journal:  Science       Date:  1990-06-01       Impact factor: 47.728

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor.

Authors:  J Kang; H G Lemaire; A Unterbeck; J M Salbaum; C L Masters; K H Grzeschik; G Multhaup; K Beyreuther; B Müller-Hill
Journal:  Nature       Date:  1987 Feb 19-25       Impact factor: 49.962

8.  Proteolytic processing of farnesylated peptides: assay and partial purification from pig brain membranes of an endopeptidase which has the characteristics of E.C. 3.4.24.15.

Authors:  T N Akopyan; Y Couedel; M Orlowski; M C Fournie-Zaluski; B P Roques
Journal:  Biochem Biophys Res Commun       Date:  1994-01-28       Impact factor: 3.575

9.  The regulation of amyloid beta protein precursor secretion and its modulatory role in cell adhesion.

Authors:  D Schubert; L W Jin; T Saitoh; G Cole
Journal:  Neuron       Date:  1989-12       Impact factor: 17.173

10.  Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.

Authors:  A Weidemann; G König; D Bunke; P Fischer; J M Salbaum; C L Masters; K Beyreuther
Journal:  Cell       Date:  1989-04-07       Impact factor: 41.582
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