Literature DB >> 18997931

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions.

Manfred T Reetz1, Sheng Wu.   

Abstract

Enantioselective mutants of the thermally robust phenyl acetone monooxygenase (PAMO) as catalysts in Baeyer-Villiger reactions have been evolved by utilizing saturation mutagenesis in which drastically reduced amino acid alphabets are employed at homologous enzyme positions.

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Year:  2008        PMID： 18997931     DOI： 10.1039/b813388c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  25 in total

1.  Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.

Authors:  Anders G Sandström; Ylva Wikmark; Karin Engström; Jonas Nyhlén; Jan-E Bäckvall
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-16       Impact factor: 11.205

Review 2.  Directed evolution drives the next generation of biocatalysts.

Authors:  Nicholas J Turner
Journal:  Nat Chem Biol       Date:  2009-08       Impact factor: 15.040

3.  Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis.

Authors:  Hanna M Dudek; Gonzalo de Gonzalo; Daniel E Torres Pazmiño; Piotr Stepniak; Lucjan S Wyrwicz; Leszek Rychlewski; Marco W Fraaije
Journal:  Appl Environ Microbiol       Date:  2011-07-01       Impact factor: 4.792

4.  Efficient Synthesis of Methyl 3-Acetoxypropionate by a Newly Identified Baeyer-Villiger Monooxygenase.

Authors:  Yuan-Yang Liu; Chun-Xiu Li; Jian-He Xu; Gao-Wei Zheng
Journal:  Appl Environ Microbiol       Date:  2019-05-16       Impact factor: 4.792

5.  A part toolbox to tune genetic expression in Bacillus subtilis.

Authors:  Sarah Guiziou; Vincent Sauveplane; Hung-Ju Chang; Caroline Clerté; Nathalie Declerck; Matthieu Jules; Jerome Bonnet
Journal:  Nucleic Acids Res       Date:  2016-07-08       Impact factor: 16.971

6.  Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453.

Authors:  Hannes Leisch; Rong Shi; Stephan Grosse; Krista Morley; Hélène Bergeron; Miroslaw Cygler; Hiroaki Iwaki; Yoshie Hasegawa; Peter C K Lau
Journal:  Appl Environ Microbiol       Date:  2012-01-20       Impact factor: 4.792

7.  Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis.

Authors:  Chao Guo; Yanpu Chen; Yu Zheng; Wei Zhang; Yunwen Tao; Juan Feng; Lixia Tang
Journal:  Appl Environ Microbiol       Date:  2015-02-13       Impact factor: 4.792

8.  SwiftLib: rapid degenerate-codon-library optimization through dynamic programming.

Authors:  Timothy M Jacobs; Hayretin Yumerefendi; Brian Kuhlman; Andrew Leaver-Fay
Journal:  Nucleic Acids Res       Date:  2014-12-24       Impact factor: 16.971

9.  Substitution of a Single Amino Acid Reverses the Regiospecificity of the Baeyer-Villiger Monooxygenase PntE in the Biosynthesis of the Antibiotic Pentalenolactone.

Authors:  Ke Chen; Shiwen Wu; Lu Zhu; Chengde Zhang; Wensheng Xiang; Zixin Deng; Haruo Ikeda; David E Cane; Dongqing Zhu
Journal:  Biochemistry       Date:  2016-11-23       Impact factor: 3.162

10.  Facilitating the Evolution of Esterase Activity from a Promiscuous Enzyme (Mhg) with Catalytic Functions of Amide Hydrolysis and Carboxylic Acid Perhydrolysis by Engineering the Substrate Entrance Tunnel.

Authors:  Xiaodan Yan; Jianjun Wang; Yu Sun; Junge Zhu; Sheng Wu
Journal:  Appl Environ Microbiol       Date:  2016-10-27       Impact factor: 4.792
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