Literature DB >> 1899567

Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.

P J Padfield1, T G Ding, J D Jamieson.   

Abstract

We have examined the influence of guanine nucleotides on Ca2(+)-dependent amylase secretion from SLO permeabilized rat pancreatic acini. GTP gamma S (100 microM) stimulated Ca2+ dependent amylase release, decreasing the EC50 for Ca2+ from 1.4 to 0.8 microM. By contrast, GDP (1mM) and dGDP (1mM) inhibited the maximal Ca2(+)-dependent secretory response. Measurement of IP3 liberation showed that Ca2+ stimulation did not increase the activity of phospholipase C (PLC) postulated to be linked to a G-protein termed Gp; GDP and dGDP must therefore be exerting their inhibitory action via a GTP-binding protein distinct from the PLC-linked Gp.

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Year:  1991        PMID： 1899567     DOI： 10.1016/0006-291x(91)91450-q

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  Association of nucleoside diphosphate kinase with pancreatic zymogen granules: effects of local GTP generation on granule membrane characteristics.

Authors:  S J Marciniak; J M Edwardson
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

2.  Cholecystokinin octapeptide inhibits Ca2+-dependent amylase secretion from permeabilized pancreatic acini by blocking the MgATP-dependent priming of exocytosis.

Authors:  P J Padfield; N Panesar
Journal:  Biochem J       Date:  1998-02-15       Impact factor: 3.857

3.  Differential effects of G-protein activators on 5-hydroxytryptamine and platelet-derived growth factor release from streptolysin-O-permeabilized human platelets.

Authors:  P J Padfield; N Panesar; P Henderson; J J Baldassare
Journal:  Biochem J       Date:  1996-02-15       Impact factor: 3.857

4.  Overexpression of Rab3D enhances regulated amylase secretion from pancreatic acini of transgenic mice.

Authors:  H Ohnishi; L C Samuelson; D I Yule; S A Ernst; J A Williams
Journal:  J Clin Invest       Date:  1997-12-15       Impact factor: 14.808

5.  Characteristics and mechanism of enzyme secretion and increase in [Ca2+]i in Saikosaponin(I) stimulated rat pancreatic acinar cells.

Authors:  Yi Yu; Wen-Xiu Yang; Hui Wang; Wen-Zheng Zhang; Bao-Hua Liu; Zhi-Yong Dong
Journal:  World J Gastroenterol       Date:  2002-06       Impact factor: 5.742

6.  Fusion between rat pancreatic zymogen granules and plasma membranes. Modulation by a GTP-binding protein.

Authors:  C M MacLean; J M Edwardson
Journal:  Biochem J       Date:  1992-09-15       Impact factor: 3.857

7.  Protein kinase A modulates Ca(2+)- and protein kinase C-dependent amylase release in permeabilized rat pancreatic acini.

Authors:  A J O'Sullivan; J D Jamieson
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

8.  A synthetic peptide of the rab3a effector domain stimulates amylase release from permeabilized pancreatic acini.

Authors:  P J Padfield; W E Balch; J D Jamieson
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

9.  Activation of protein kinase C is not an absolute requirement for amylase release from permeabilized rat pancreatic acini.

Authors:  A J O'Sullivan; J D Jamieson
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

10.  Pancreatic plasma membranes: promiscuous partners in membrane fusion.

Authors:  E G Lee; S J Marciniak; C M MacLean; J M Edwardson
Journal:  Biochem J       Date:  1994-03-15       Impact factor: 3.857
  10 in total

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