Literature DB >> 8141772

Pancreatic plasma membranes: promiscuous partners in membrane fusion.

E G Lee1, S J Marciniak, C M MacLean, J M Edwardson.   

Abstract

We have developed a system in which the fusion of pancreatic plasma membranes with zymogen granules can be studied in vitro. We show here that pancreatic plasma membranes fuse not only with pancreatic zymogen granules but also with parotid secretory granules. In contrast, parotid membranes fuse only with parotid granules and not with pancreatic granules. The extent of fusion is insensitive to Ca2+ for all combinations of plasma membranes and granules. Guanosine 5'-[gamma-thio]triphosphate (GTP[S]), on the other hand, stimulates fusion of pancreatic membranes with both pancreatic granules and parotid granules, but inhibits fusion between parotid membranes and parotid granules.

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Year:  1994        PMID： 8141772      PMCID： PMC1137901          DOI： 10.1042/bj2980599

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Rat pancreatic acini permeabilised with streptolysin O secrete amylase at Ca2+ concentrations in the micromolar range, when provided with ATP and GTP gamma S.

Authors:  J M Edwardson; C Vickery; L J Christy
Journal:  Biochim Biophys Acta       Date:  1990-06-12

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.

Authors:  A Fabiato; F Fabiato
Journal:  J Physiol (Paris)       Date:  1979

4.  Interaction of gamma-glutamyl transpeptidase with amino acids, dipeptides, and derivatives and analogs of glutathione.

Authors:  S S Tate; A Meister
Journal:  J Biol Chem       Date:  1974-12-10       Impact factor: 5.157

5.  Purification of a plasma membrane-bound adenosine triphosphatase from plant roots.

Authors:  T K Hodges; R T Leonard
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  A new method for the determination of alpha-amylase.

Authors:  H Rinderknecht; P Wilding; B J Haverback
Journal:  Experientia       Date:  1967-10-15

7.  Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.

Authors:  P J Padfield; T G Ding; J D Jamieson
Journal:  Biochem Biophys Res Commun       Date:  1991-01-31       Impact factor: 3.575

8.  A specific interaction in vitro between pancreatic zymogen granules and plasma membranes: stimulation by G-protein activators but not by Ca2+.

Authors:  C Y Nadin; J Rogers; S Tomlinson; J M Edwardson
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539

9.  Involvement of a phosphoprotein on the zymogen granule membrane in the control of regulated exocytosis in the exocrine pancreas.

Authors:  C M MacLean; S J Marciniak; D V Hall; J M Edwardson
Journal:  J Cell Sci       Date:  1993-10       Impact factor: 5.285

10.  Plasma membrane of the rat parotid gland: preparation and partial characterization of a fraction containing the secretory surface.

Authors:  P Arvan; J D Castle
Journal:  J Cell Biol       Date:  1982-10       Impact factor: 10.539
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  2 in total

1.  Identification of a lysosomal protein causing lipid transfer, using a fluorescence assay designed to monitor membrane fusion between rat liver endosomes and lysosomes.

Authors:  T Kuwana; B M Mullock; J P Luzio
Journal:  Biochem J       Date:  1995-06-15       Impact factor: 3.857

2.  Ca(2+)-independent fusion of secretory granules with phospholipase A2-treated plasma membranes in vitro.

Authors:  T Nagao; T Kubo; R Fujimoto; H Nishio; T Takeuchi; F Hata
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857
  2 in total

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