Literature DB >> 18988021

A family of LIC vectors for high-throughput cloning and purification of proteins.

William H Eschenfeldt1, Stols Lucy, Cynthia Sanville Millard, Andrzej Joachimiak, I Donnelly Mark.   

Abstract

Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate pro tein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we describe here.

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Year:  2009        PMID： 18988021      PMCID： PMC2771622          DOI： 10.1007/978-1-59745-196-3_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

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Journal:  Protein Expr Purif       Date:  2002-06       Impact factor: 1.650

4.  High throughput methods for gene cloning and expression.

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Journal:  Protein Expr Purif       Date:  2002-06       Impact factor: 1.650

5.  New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols.

Authors:  Lucy Stols; Min Zhou; William H Eschenfeldt; Cynthia Sanville Millard; James Abdullah; Frank R Collart; Youngchang Kim; Mark I Donnelly
Journal:  Protein Expr Purif       Date:  2007-02-06       Impact factor: 1.650

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Authors:  L J Dieckman; W Zhang; D J Rodi; M I Donnelly; F R Collart
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8.  Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader peptide derived from the GroES cochaperone.

Authors:  M I Donnelly; P W Stevens; L Stols; S X Su; S Tollaksen; C Giometti; A Joachimiak
Journal:  Protein Expr Purif       Date:  2001-08       Impact factor: 1.650

9.  Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro.

Authors:  Sreedevi Nallamsetty; Rachel B Kapust; József Tözsér; Scott Cherry; Joseph E Tropea; Terry D Copeland; David S Waugh
Journal:  Protein Expr Purif       Date:  2004-11       Impact factor: 1.650

10.  An expression vector tailored for large-scale, high-throughput purification of recombinant proteins.

Authors:  Mark I Donnelly; Min Zhou; Cynthia Sanville Millard; Shonda Clancy; Lucy Stols; William H Eschenfeldt; Frank R Collart; Andrzej Joachimiak
Journal:  Protein Expr Purif       Date:  2006-01-30       Impact factor: 1.650
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5.  Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana.

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6.  Ctp1 protein-DNA filaments promote DNA bridging and DNA double-strand break repair.

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Journal:  J Biol Chem       Date:  2019-01-09       Impact factor: 5.157

7.  Structural and functional plasticity of antibiotic resistance nucleotidylyltransferases revealed by molecular characterization of lincosamide nucleotidylyltransferases lnu(A) and lnu(D).

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Journal:  J Mol Biol       Date:  2015-04-18       Impact factor: 5.469

Review 8.  High-throughput cloning and expression library creation for functional proteomics.

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Journal:  Proteomics       Date:  2013-04-05       Impact factor: 3.984

9.  NOT2 proteins promote polymerase II-dependent transcription and interact with multiple MicroRNA biogenesis factors in Arabidopsis.

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10.  Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor.

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