BACKGROUND: Immunoglobulin A (IgA) autoantibodies to tissue transglutaminase (tTG) are commonly used for screening and diagnosing of celiac disease (CD). Seroreactivity for anti-Saccharomyces cerevisiae antibody (ASCA) and bacterial antigens have also been detected in CD patients. The aim of this study was to examine prospectively serologic responses to microbial targets in adult CD patients at the time of diagnosis and during a gluten-free diet (GFD). Further, we wanted to evaluate whether these serologic specificities could provide new tools for the follow-up of CD patients. METHODS: Data on 55 adult biopsy-proven CD patients were available for follow-up study. Upper gastrointestinal endoscopy was performed on all patients. Sera from patients were tested for antibodies to tTG and ASCA and additionally analyzed with IgA enzyme-linked immunosorbent assays to Pseudomonas fluorescens-associated sequence, I2, and to a Bacteroides caccae TonB-linked outer membrane protein, OmpW. RESULTS: At the time of diagnosis, 91% of CD cases were positive for tTG and 49% for ASCA; positive seroreactivity to I2 was found in 86% and to OmpW in 60% of CD patients at the time of diagnosis. The frequency of seropositivity and serum levels of these antibodies decreased during GFD. Moreover, we found that the decline in the serum levels was significant in all of these markers (p < 0.005). Interestingly, we also found that serum levels of ASCA correlated with the grade of mucosal morphology (p = 0.021), as the ASCA serum levels declined in accordance with mucosal healing. CONCLUSIONS: Commensal enteric bacteria seem to play a role in the small intestinal mucosal damage in CD. This was proven by the serological responses to different microbial antigens shown in this study. Serum levels of ASCA, anti-I2, and anti-OmpW antibodies decreased significantly during GFD, indicating that these serologic markers are gluten dependent in CD patients. These specificities could provide new tools in the follow-up of CD patients.
BACKGROUND:Immunoglobulin A (IgA) autoantibodies to tissue transglutaminase (tTG) are commonly used for screening and diagnosing of celiac disease (CD). Seroreactivity for anti-Saccharomyces cerevisiae antibody (ASCA) and bacterial antigens have also been detected in CDpatients. The aim of this study was to examine prospectively serologic responses to microbial targets in adult CDpatients at the time of diagnosis and during a gluten-free diet (GFD). Further, we wanted to evaluate whether these serologic specificities could provide new tools for the follow-up of CDpatients. METHODS: Data on 55 adult biopsy-proven CDpatients were available for follow-up study. Upper gastrointestinal endoscopy was performed on all patients. Sera from patients were tested for antibodies to tTG and ASCA and additionally analyzed with IgA enzyme-linked immunosorbent assays to Pseudomonas fluorescens-associated sequence, I2, and to a Bacteroides caccae TonB-linked outer membrane protein, OmpW. RESULTS: At the time of diagnosis, 91% of CD cases were positive for tTG and 49% for ASCA; positive seroreactivity to I2 was found in 86% and to OmpW in 60% of CDpatients at the time of diagnosis. The frequency of seropositivity and serum levels of these antibodies decreased during GFD. Moreover, we found that the decline in the serum levels was significant in all of these markers (p < 0.005). Interestingly, we also found that serum levels of ASCA correlated with the grade of mucosal morphology (p = 0.021), as the ASCA serum levels declined in accordance with mucosal healing. CONCLUSIONS: Commensal enteric bacteria seem to play a role in the small intestinal mucosal damage in CD. This was proven by the serological responses to different microbial antigens shown in this study. Serum levels of ASCA, anti-I2, and anti-OmpW antibodies decreased significantly during GFD, indicating that these serologic markers are gluten dependent in CDpatients. These specificities could provide new tools in the follow-up of CDpatients.
Authors: A Granito; D Zauli; P Muratori; L Muratori; A Grassi; R Bortolotti; N Petrolini; L Veronesi; P Gionchetti; F B Bianchi; U Volta Journal: Aliment Pharmacol Ther Date: 2005-04-01 Impact factor: 8.171
Authors: M Peeters; B Geypens; D Claus; H Nevens; Y Ghoos; G Verbeke; F Baert; S Vermeire; R Vlietinck; P Rutgeerts Journal: Gastroenterology Date: 1997-09 Impact factor: 22.682
Authors: Jan G M C Damoiseaux; Bas Bouten; Annick M L W Linders; Jos Austen; Caroline Roozendaal; Maurice G V M Russel; Pierre-Philippe Forget; Jan Willem Cohen Tervaert Journal: J Clin Immunol Date: 2002-09 Impact factor: 8.317
Authors: Sara Ashorn; Hanna Raukola; Tuuli Välineva; Merja Ashorn; Bo Wei; Jonathan Braun; Immo Rantala; Katri Kaukinen; Tiina Luukkaala; Pekka Collin; Markku Mäki; Sari Iltanen Journal: J Clin Immunol Date: 2008-05-22 Impact factor: 8.317
Authors: M G Clemente; S De Virgiliis; J S Kang; R Macatagney; M P Musu; M R Di Pierro; S Drago; M Congia; A Fasano Journal: Gut Date: 2003-02 Impact factor: 23.059
Authors: Manuel A Silva; Jennifer Jury; Yolanda Sanz; Michelle Wiepjes; Xianxi Huang; Joseph A Murray; Chella S David; Alessio Fasano; Elena F Verdú Journal: Dig Dis Sci Date: 2011-08-07 Impact factor: 3.199
Authors: Emily G Severance; Armin Alaedini; Shuojia Yang; Meredith Halling; Kristin L Gressitt; Cassie R Stallings; Andrea E Origoni; Crystal Vaughan; Sunil Khushalani; F Markus Leweke; Faith B Dickerson; Robert H Yolken Journal: Schizophr Res Date: 2012-03-24 Impact factor: 4.939