BACKGROUND: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays. OBJECTIVES: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses. STUDY DESIGN: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed. RESULTS: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV. CONCLUSIONS: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
BACKGROUND: Three new humanpolyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays. OBJECTIVES: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses. STUDY DESIGN: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed. RESULTS: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV. CONCLUSIONS: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
Authors: Seweryn Bialasiewicz; Rebecca Rockett; David W Whiley; Yacine Abed; Tobias Allander; Michael Binks; Guy Boivin; Allen C Cheng; Ju-Young Chung; Patricia E Ferguson; Nicole M Gilroy; Amanda J Leach; Cecilia Lindau; John W Rossen; Tania C Sorrell; Michael D Nissen; Theo P Sloots Journal: J Virol Date: 2010-03-31 Impact factor: 5.103
Authors: Massimiliano Bergallo; Maria Elena Terlizzi; Sara Astegiano; Marco Ciotti; Muhammed Babakir-Mina; Carlo Federico Perno; Rossana Cavallo; Cristina Costa Journal: J Virol Methods Date: 2009-07-29 Impact factor: 2.014
Authors: S D Thiberville; L Ninove; V Vu Hai; E Botelho-Nevers; C Gazin; L Thirion; N Salez; X de Lamballerie; R Charrel; P Brouqui Journal: J Med Virol Date: 2012-07 Impact factor: 2.327