Literature DB >> 18953349

A noncytotoxic DsRed variant for whole-cell labeling.

Rita L Strack1, Daniel E Strongin, Dibyendu Bhattacharyya, Wen Tao, Allison Berman, Hal E Broxmeyer, Robert J Keenan, Benjamin S Glick.   

Abstract

A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

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Year:  2008        PMID： 18953349      PMCID： PMC4107390          DOI： 10.1038/nmeth.1264

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed).

Authors:  Brooke J Bevis; Benjamin S Glick
Journal:  Nat Biotechnol       Date:  2002-01       Impact factor: 54.908

Review 2.  Advances in fluorescent protein technology.

Authors:  Nathan C Shaner; George H Patterson; Michael W Davidson
Journal:  J Cell Sci       Date:  2007-12-15       Impact factor: 5.285

3.  Bright monomeric red fluorescent protein with an extended fluorescence lifetime.

Authors:  Ekaterina M Merzlyak; Joachim Goedhart; Dmitry Shcherbo; Mariya E Bulina; Aleksandr S Shcheglov; Arkady F Fradkov; Anna Gaintzeva; Konstantin A Lukyanov; Sergey Lukyanov; Theodorus W J Gadella; Dmitriy M Chudakov
Journal:  Nat Methods       Date:  2007-06-17       Impact factor: 28.547

4.  Optimized and far-red-emitting variants of fluorescent protein eqFP611.

Authors:  Simone Kredel; Karin Nienhaus; Franz Oswald; Michael Wolff; Sergey Ivanchenko; Florian Cymer; Andreas Jeromin; Francois J Michel; Klaus-Dieter Spindler; Ralf Heilker; G Ulrich Nienhaus; Jörg Wiedenmann
Journal:  Chem Biol       Date:  2008-03

5.  Improving the photostability of bright monomeric orange and red fluorescent proteins.

Authors:  Nathan C Shaner; Michael Z Lin; Michael R McKeown; Paul A Steinbach; Kristin L Hazelwood; Michael W Davidson; Roger Y Tsien
Journal:  Nat Methods       Date:  2008-05-04       Impact factor: 28.547

6.  Enhanced green fluorescent protein is a nearly ideal long-term expression tracer for hematopoietic stem cells, whereas DsRed-express fluorescent protein is not.

Authors:  Wen Tao; Barbara-Graham Evans; Jing Yao; Scott Cooper; Kenneth Cornetta; Christopher B Ballas; Giao Hangoc; Hal E Broxmeyer
Journal:  Stem Cells       Date:  2006-11-30       Impact factor: 6.277

7.  Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.

Authors:  Daniel E Strongin; Brooke Bevis; Nhi Khuong; Maureen E Downing; Rita L Strack; Karthik Sundaram; Benjamin S Glick; Robert J Keenan
Journal:  Protein Eng Des Sel       Date:  2007-10-25       Impact factor: 1.650

8.  The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ.

Authors:  Rita De Gasperi; Anne B Rocher; Miguel A Gama Sosa; Susan L Wearne; Gissel M Perez; Victor L Friedrich; Patrick R Hof; Gregory A Elder
Journal:  Genesis       Date:  2008-06       Impact factor: 2.487

9.  Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.

Authors:  Asako Sakaue-Sawano; Hiroshi Kurokawa; Toshifumi Morimura; Aki Hanyu; Hiroshi Hama; Hatsuki Osawa; Saori Kashiwagi; Kiyoko Fukami; Takaki Miyata; Hiroyuki Miyoshi; Takeshi Imamura; Masaharu Ogawa; Hisao Masai; Atsushi Miyawaki
Journal:  Cell       Date:  2008-02-08       Impact factor: 41.582

Review 10.  Gene delivery by lentivirus vectors.

Authors:  Adam S Cockrell; Tal Kafri
Journal:  Mol Biotechnol       Date:  2007-07       Impact factor: 2.860
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  97 in total

1.  Chromophore formation in DsRed occurs by a branched pathway.

Authors:  Rita L Strack; Daniel E Strongin; Laurens Mets; Benjamin S Glick; Robert J Keenan
Journal:  J Am Chem Soc       Date:  2010-06-23       Impact factor: 15.419

2.  Engineered fluorescent proteins: innovations and applications.

Authors:  Michael W Davidson; Robert E Campbell
Journal:  Nat Methods       Date:  2009-10       Impact factor: 28.547

Review 3.  Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.

Authors:  Robert H Newman; Matthew D Fosbrink; Jin Zhang
Journal:  Chem Rev       Date:  2011-04-01       Impact factor: 60.622

4.  Magnetic nanosensors optimized for rapid and reversible self-assembly.

Authors:  Elisenda Rodriguez; Victor S Lelyveld; Tatjana Atanasijevic; Satoshi Okada; Alan Jasanoff
Journal:  Chem Commun (Camb)       Date:  2014-02-24       Impact factor: 6.222

Review 5.  The fluorescent protein palette: tools for cellular imaging.

Authors:  Richard N Day; Michael W Davidson
Journal:  Chem Soc Rev       Date:  2009-08-04       Impact factor: 54.564

6.  Rapid and sensitive lentivirus vector-based conditional gene expression assay to monitor and quantify cell fusion activity.

Authors:  Manuel A F V Gonçalves; Josephine M Janssen; Maarten Holkers; Antoine A F de Vries
Journal:  PLoS One       Date:  2010-06-03       Impact factor: 3.240

7.  Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila.

Authors:  Lydia J Bright; Nichole Kambesis; Scott Brent Nelson; Byeongmoon Jeong; Aaron P Turkewitz
Journal:  PLoS Genet       Date:  2010-10-14       Impact factor: 5.917

8.  A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.

Authors:  Rita L Strack; Birka Hein; Dibyendu Bhattacharyya; Stefan W Hell; Robert J Keenan; Benjamin S Glick
Journal:  Biochemistry       Date:  2009-09-08       Impact factor: 3.162

9.  Red fluorescent Xenopus laevis: a new tool for grafting analysis.

Authors:  Christoph Waldner; Magdalena Roose; Gerhart U Ryffel
Journal:  BMC Dev Biol       Date:  2009-06-23       Impact factor: 1.978

10.  Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.

Authors:  Rita L Strack; Dibyendu Bhattacharyya; Benjamin S Glick; Robert J Keenan
Journal:  BMC Biotechnol       Date:  2009-04-03       Impact factor: 2.563
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