Literature DB >> 17572680

Bright monomeric red fluorescent protein with an extended fluorescence lifetime.

Ekaterina M Merzlyak1, Joachim Goedhart, Dmitry Shcherbo, Mariya E Bulina, Aleksandr S Shcheglov, Arkady F Fradkov, Anna Gaintzeva, Konstantin A Lukyanov, Sergey Lukyanov, Theodorus W J Gadella, Dmitriy M Chudakov.   

Abstract

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

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Year:  2007        PMID： 17572680     DOI： 10.1038/nmeth1062

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  271 in total

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