Literature DB >> 1892862

A method for preparing analytically pure sodium dithionite. Dithionite quality and observed nitrogenase-specific activities.

C E McKenna1, W G Gutheil, W Song.   

Abstract

Sodium dithionite (Na2S2O4) is widely used as a reductant in biochemical studies, but has not been available in its pure form. A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions. Twice-recrystallized dithionite had a purity of 99 +/- 1% by UV spectroscopy (A315) and elemental analysis. The influence of dithionite quality on the apparent reduction activities of the nitrogenase components (Av1 and Av2) from Azotobacter vinelandii was investigated.

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Year:  1991        PMID： 1892862     DOI： 10.1016/0304-4165(91)90082-r

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  18 in total

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