Literature DB >> 1886782

General method for direct cloning of DNA fragments generated by the polymerase chain reaction.

D Kovalic1, J H Kwak, B Weisblum.   

Abstract

Mesh:

Substances:

Year:  1991        PMID: 1886782      PMCID: PMC328657          DOI: 10.1093/nar/19.16.4560

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


× No keyword cloud information.
  6 in total

1.  A universal method for the direct cloning of PCR amplified nucleic acid.

Authors:  D A Mead; N K Pey; C Herrnstadt; R A Marcil; L M Smith
Journal:  Biotechnology (N Y)       Date:  1991-07

2.  Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Authors:  D Marchuk; M Drumm; A Saulino; F S Collins
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

3.  A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.

Authors:  T A Holton; M W Graham
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

4.  Restriction endonuclease cleavage at the termini of PCR products.

Authors:  D L Kaufman; G A Evans
Journal:  Biotechniques       Date:  1990-09       Impact factor: 1.993

5.  Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites.

Authors:  V Jung; S B Pestka; S Pestka
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

6.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

Authors:  J M Clark
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

  6 in total
  51 in total

1.  pSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease.

Authors:  Svetoslav G Dimov
Journal:  Mol Biol Rep       Date:  2011-12-28       Impact factor: 2.316

2.  pYEMF, a pUC18-derived XcmI T-vector for efficient cloning of PCR products.

Authors:  Jingsong Gu; Chunjiang Ye
Journal:  Mol Biotechnol       Date:  2011-03       Impact factor: 2.695

Review 3.  Genotypic microbial community profiling: a critical technical review.

Authors:  Andreas Nocker; Mark Burr; Anne K Camper
Journal:  Microb Ecol       Date:  2007-03-08       Impact factor: 4.552

4.  One-step preparation of a TA-cloning vector from a specially designed parent plasmid containing a dual lacZ gene system.

Authors:  Soo Youn Jun; Seong Jun Yoon; Sang Hyeon Kang
Journal:  Mol Biotechnol       Date:  2010-05       Impact factor: 2.695

5.  Molecular and biochemical characterisation of DNA-dependent protein kinase-defective rodent mutant irs-20.

Authors:  A Priestley; H J Beamish; D Gell; A G Amatucci; M C Muhlmann-Diaz; B K Singleton; G C Smith; T Blunt; L C Schalkwyk; J S Bedford; S P Jackson; P A Jeggo; G E Taccioli
Journal:  Nucleic Acids Res       Date:  1998-04-15       Impact factor: 16.971

6.  Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein.

Authors:  S Y Choi
Journal:  Plant Mol Biol       Date:  1996-12       Impact factor: 4.076

7.  Structure-function studies of the adenylate cyclase toxin of Bordetella pertussis and the leukotoxin of Pasteurella haemolytica by heterologous C protein activation and construction of hybrid proteins.

Authors:  G Westrop; K Hormozi; N da Costa; R Parton; J Coote
Journal:  J Bacteriol       Date:  1997-02       Impact factor: 3.490

8.  Molecular and biochemical characterization of xrs mutants defective in Ku80.

Authors:  B K Singleton; A Priestley; H Steingrimsdottir; D Gell; T Blunt; S P Jackson; A R Lehmann; P A Jeggo
Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

9.  The U3 promoter region of the acutely lethal simian immunodeficiency virus clone smmPBj1.9 confers related biological activity on the apathogenic clone agm3mc.

Authors:  M T Dittmar; K Cichutek; P N Fultz; R Kurth
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-28       Impact factor: 11.205

10.  Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.

Authors:  T Blunt; D Gell; M Fox; G E Taccioli; A R Lehmann; S P Jackson; P A Jeggo
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.