Literature DB >> 1885537

Escherichia coli orfE (upstream of pyrE) encodes RNase PH.

K A Ost1, M P Deutscher.   

Abstract

RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

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Year:  1991        PMID： 1885537      PMCID： PMC208279          DOI： 10.1128/jb.173.17.5589-5591.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

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Authors:  F Bonekamp; H D Andersen; T Christensen; K F Jensen
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

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4.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
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5.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  Molecular and mutational analysis of three genes preceding pyrE on the Escherichia coli chromosome.

Authors:  P Poulsen; J T Andersen; K F Jensen
Journal:  Mol Microbiol       Date:  1989-03       Impact factor: 3.501

7.  3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement.

Authors:  H Cudny; M P Deutscher
Journal:  J Biol Chem       Date:  1988-01-25       Impact factor: 5.157

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Authors:  P Poulsen; K F Jensen; P Valentin-Hansen; P Carlsson; L G Lundberg
Journal:  Eur J Biochem       Date:  1983-09-15

9.  Mechanism of UTP-modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription.

Authors:  F Bonekamp; K Clemmesen; O Karlström; K F Jensen
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598

10.  Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attentuation.

Authors:  P Poulsen; F Bonekamp; K F Jensen
Journal:  EMBO J       Date:  1984-08       Impact factor: 11.598
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  8 in total

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Review 5.  Promiscuous exoribonucleases of Escherichia coli.

Authors:  M P Deutscher
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

6.  Identification of the rph (RNase PH) gene of Bacillus subtilis: evidence for suppression of cold-sensitive mutations in Escherichia coli.

Authors:  M G Craven; D J Henner; D Alessi; A T Schauer; K A Ost; M P Deutscher; D I Friedman
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Review 7.  Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.

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Review 8.  Bacterial ribonucleases and their roles in RNA metabolism.

Authors:  David H Bechhofer; Murray P Deutscher
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  8 in total

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