Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

Literature DB >> 1885532

Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

S K Roof¹, J D Allard, K P Bertrand, K Postle.

Abstract

Alkaline phosphatase (PhoA) fusions to TonB amino acids 32, 60, 125, 207, and 239 (the carboxy terminus) all showed high PhoA activity; a PhoA fusion to TonB amino acid 12 was inactive. The full-length TonB-PhoA fusion protein was associated with the cytoplasmic membrane and retained partial TonB function. These results support a model in which TonB is anchored in the cytoplasmic membrane by its hydrophobic amino terminus, with the remainder of the protein, including its hydrophobic carboxy terminus, extending into the periplasm.

Entities: Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1885532 PMCID： PMC208271 DOI： 10.1128/jb.173.17.5554-5557.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

20 in total

1. Identification of an Escherichia coli inner membrane polypeptide specified by a lambda-tonB transducing.

Authors: G S Plastow; I B Holland
Journal: Biochem Biophys Res Commun Date: 1979-10-12 Impact factor: 3.575

2. lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

Authors: J Calamia; C Manoil
Journal: Proc Natl Acad Sci U S A Date: 1990-07 Impact factor: 11.205

3. Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB.

Authors: E Fischer; K Günter; V Braun
Journal: J Bacteriol Date: 1989-09 Impact factor: 3.490

4. Determinants of membrane protein topology.

Authors: D Boyd; C Manoil; J Beckwith
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

5. Escherichia coli TonB protein is exported from the cytoplasm without proteolytic cleavage of its amino terminus.

Authors: K Postle; J T Skare
Journal: J Biol Chem Date: 1988-08-05 Impact factor: 5.157

6. A comprehensive set of sequence analysis programs for the VAX.

Authors: J Devereux; P Haeberli; O Smithies
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

7. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

Authors: S Michaelis; H Inouye; D Oliver; J Beckwith
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

8. DNA sequence of the Escherichia coli tonB gene.

Authors: K Postle; R F Good
Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205

9. Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression.

Authors: H S Moyed; T T Nguyen; K P Bertrand
Journal: J Bacteriol Date: 1983-08 Impact factor: 3.490

10. The C terminus of penicillin-binding protein 5 is essential for localisation to the E. coli inner membrane.

Authors: J M Pratt; M E Jackson; I B Holland
Journal: EMBO J Date: 1986-09 Impact factor: 11.598

37 in total

1. Characterization of in vitro interactions between a truncated TonB protein from Escherichia coli and the outer membrane receptors FhuA and FepA.

Authors: G S Moeck; L Letellier
Journal: J Bacteriol Date: 2001-05 Impact factor: 3.490

2. Bordetella pertussis TonB, a Bvg-independent virulence determinant.

Authors: E Pradel; N Guiso; F D Menozzi; C Locht
Journal: Infect Immun Date: 2000-04 Impact factor: 3.441

3. Differential effects of mutations in tonB1 on intrinsic multidrug resistance and iron acquisition in Pseudomonas aeruginosa.

Authors: Qixun Zhao; Keith Poole
Journal: J Bacteriol Date: 2002-04 Impact factor: 3.490

Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

1. Identification of an Escherichia coli inner membrane polypeptide specified by a lambda-tonB transducing.

2. lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

3. Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB.

4. Determinants of membrane protein topology.

5. Escherichia coli TonB protein is exported from the cytoplasm without proteolytic cleavage of its amino terminus.

6. A comprehensive set of sequence analysis programs for the VAX.

7. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

8. DNA sequence of the Escherichia coli tonB gene.

9. Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression.

10. The C terminus of penicillin-binding protein 5 is essential for localisation to the E. coli inner membrane.

1. Characterization of in vitro interactions between a truncated TonB protein from Escherichia coli and the outer membrane receptors FhuA and FepA.

2. Bordetella pertussis TonB, a Bvg-independent virulence determinant.

3. Differential effects of mutations in tonB1 on intrinsic multidrug resistance and iron acquisition in Pseudomonas aeruginosa.

4. Sequence changes in the ton box region of BtuB affect its transport activities and interaction with TonB protein.

5. Identification of functionally important TonB-ExbD periplasmic domain interactions in vivo.

6. The same periplasmic ExbD residues mediate in vivo interactions between ExbD homodimers and ExbD-TonB heterodimers.

7. Membrane topology of the Escherichia coli ExbD protein.

8. His(20) provides the sole functionally significant side chain in the essential TonB transmembrane domain.

9. Interactions of the energy transducer TonB with noncognate energy-harvesting complexes.

10. Deletion and substitution analysis of the Escherichia coli TonB Q160 region.