| Literature DB >> 18851948 |
Rodrigo Morales1, Kimberley A Buytaert-Hoefen2, Dennisse Gonzalez-Romero3, Joaquin Castilla3, Eric T Hansen2, Dennis Hlavinka2, Raymond P Goodrich2, Claudio Soto4.
Abstract
The link between a new variant form of Creutzfeldt-Jakob disease (vCJD) and the consumption of prion contaminated cattle meat as well as recent findings showing that vCJD can be transmitted by blood transfusion have raised public health concerns. Currently, a reliable test to identify prions in blood samples is not available. The purpose of this study was to evaluate the possibility to remove scrapie prion protein (PrP(Sc)) and infectivity from red blood cell (RBC) suspensions by a simple washing procedure using a cell separation and washing device. The extent of prion removal was assessed by Western blot, PMCA and infectivity bioassays. Our results revealed a substantial removal of infectious prions (3 logs of infectivity) by all techniques used. These data suggest that a significant amount of infectivity present in RBC preparations can be removed by a simple washing procedure. This technology may lead to increased safety of blood products and reduce the risk of further propagation of prion diseases.Entities:
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Year: 2008 PMID： 18851948 PMCID： PMC2606671 DOI： 10.1016/j.bbrc.2008.09.141
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575