Literature DB >> 20512142

Estimating prion concentration in fluids and tissues by quantitative PMCA.

Baian Chen1, Rodrigo Morales, Marcelo A Barria, Claudio Soto.   

Abstract

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

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Year:  2010        PMID： 20512142      PMCID： PMC4049222          DOI： 10.1038/nmeth.1465

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding.

Authors:  G P Saborio; B Permanne; C Soto
Journal:  Nature       Date:  2001-06-14       Impact factor: 49.962

2.  Cyclic amplification of protein misfolding and aggregation.

Authors:  Paula Saá; Joaquín Castilla; Claudio Soto
Journal:  Methods Mol Biol       Date:  2005

3.  Presymptomatic detection of prions in blood.

Authors:  Paula Saá; Joaquín Castilla; Claudio Soto
Journal:  Science       Date:  2006-07-07       Impact factor: 47.728

Review 4.  Protein misfolding cyclic amplification for diagnosis and prion propagation studies.

Authors:  Joaquín Castilla; Paula Saá; Rodrigo Morales; Karim Abid; Kinsey Maundrell; Claudio Soto
Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

5.  Tissue distribution of protease resistant prion protein in variant Creutzfeldt-Jakob disease using a highly sensitive immunoblotting assay.

Authors:  J D Wadsworth; S Joiner; A F Hill; T A Campbell; M Desbruslais; P J Luthert; J Collinge
Journal:  Lancet       Date:  2001-07-21       Impact factor: 79.321

6.  Ultra-efficient replication of infectious prions by automated protein misfolding cyclic amplification.

Authors:  Paula Saá; Joaquín Castilla; Claudio Soto
Journal:  J Biol Chem       Date:  2006-09-18       Impact factor: 5.157

7.  Detection of prions in blood.

Authors:  Joaquín Castilla; Paula Saá; Claudio Soto
Journal:  Nat Med       Date:  2005-08-28       Impact factor: 53.440

8.  In vitro generation of infectious scrapie prions.

Authors:  Joaquín Castilla; Paula Saá; Claudio Hetz; Claudio Soto
Journal:  Cell       Date:  2005-04-22       Impact factor: 41.582

9.  Urinary excretion and blood level of prions in scrapie-infected hamsters.

Authors:  Yuichi Murayama; Miyako Yoshioka; Hiroyuki Okada; Masuhiro Takata; Takashi Yokoyama; Shirou Mohri
Journal:  J Gen Virol       Date:  2007-10       Impact factor: 3.891

Review 10.  Prions: protein aggregation and infectious diseases.

Authors:  Adriano Aguzzi; Anna Maria Calella
Journal:  Physiol Rev       Date:  2009-10       Impact factor: 37.312
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  52 in total

Review 1.  New generation QuIC assays for prion seeding activity.

Authors:  Christina D Orrù; Jason M Wilham; Sarah Vascellari; Andrew G Hughson; Byron Caughey
Journal:  Prion       Date:  2012-04-01       Impact factor: 3.931

2.  Lack of prion transmission by sexual or parental routes in experimentally infected hamsters.

Authors:  Rodrigo Morales; Sandra Pritzkow; Ping Ping Hu; Claudia Duran-Aniotz; Claudio Soto
Journal:  Prion       Date:  2013-10-11       Impact factor: 3.931

3.  Superparamagnetic nanoparticle capture of prions for amplification.

Authors:  Michael B Miller; Surachai Supattapone
Journal:  J Virol       Date:  2011-01-12       Impact factor: 5.103

4.  An enzymatic treatment of soil-bound prions effectively inhibits replication.

Authors:  Samuel E Saunders; Jason C Bartz; Kurt C Vercauteren; Shannon L Bartelt-Hunt
Journal:  Appl Environ Microbiol       Date:  2011-05-13       Impact factor: 4.792

5.  The real-time quaking-induced conversion assay for detection of human prion disease and study of other protein misfolding diseases.

Authors:  Matthias Schmitz; Maria Cramm; Franc Llorens; Dominik Müller-Cramm; Steven Collins; Ryuichiro Atarashi; Katsuya Satoh; Christina D Orrù; Bradley R Groveman; Saima Zafar; Walter J Schulz-Schaeffer; Byron Caughey; Inga Zerr
Journal:  Nat Protoc       Date:  2016-10-13       Impact factor: 13.491

6.  Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion.

Authors:  Nathaniel D Denkers; Davin M Henderson; Candace K Mathiason; Edward A Hoover
Journal:  J Gen Virol       Date:  2016-05-27       Impact factor: 3.891

Review 7.  Prions: Beyond a Single Protein.

Authors:  Alvin S Das; Wen-Quan Zou
Journal:  Clin Microbiol Rev       Date:  2016-07       Impact factor: 26.132

8.  Design, implementation, and interpretation of amplification studies for prion detection.

Authors:  Nicholas J Haley; Jürgen A Richt; Kristen A Davenport; Davin M Henderson; Edward A Hoover; Matteo Manca; Byron Caughey; Douglas Marthaler; Jason Bartz; Sabine Gilch
Journal:  Prion       Date:  2018-03-09       Impact factor: 3.931

9.  Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays.

Authors:  Jason M Wilham; Christina D Orrú; Richard A Bessen; Ryuichiro Atarashi; Kazunori Sano; Brent Race; Kimberly D Meade-White; Lara M Taubner; Andrew Timmes; Byron Caughey
Journal:  PLoS Pathog       Date:  2010-12-02       Impact factor: 6.823

10.  Protein misfolding cyclic amplification of infectious prions.

Authors:  Rodrigo Morales; Claudia Duran-Aniotz; Rodrigo Diaz-Espinoza; Manuel V Camacho; Claudio Soto
Journal:  Nat Protoc       Date:  2012-06-28       Impact factor: 13.491
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