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Literature DB >> 18849422

Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB.

Jürgen A Bohnert¹, Sabine Schuster, Markus A Seeger, Eva Fähnrich, Klaas M Pos, Winfried V Kern.

Abstract

The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while other targeted mutations, including conversion of phenylalanines 136, 178, 615, 617, and 628 to alanine, had smaller and more variable effects.

Entities: Chemical Gene Mutation Species

Mesh：

Substances：

Year: 2008 PMID： 18849422 PMCID： PMC2593224 DOI： 10.1128/JB.00912-08

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. On the mechanism of substrate specificity by resistance nodulation division (RND)-type multidrug resistance pumps: the large periplasmic loops of MexD from Pseudomonas aeruginosa are involved in substrate recognition.

Authors: Weimin Mao; Mark S Warren; Deborah S Black; Takahumi Satou; Takeshi Murata; Takeshi Nishino; Naomasa Gotoh; Olga Lomovskaya
Journal: Mol Microbiol Date: 2002-11 Impact factor: 3.501

2. Selected arylpiperazines are capable of reversing multidrug resistance in Escherichia coli overexpressing RND efflux pumps.

Authors: Jürgen A Bohnert; Winfried V Kern
Journal: Antimicrob Agents Chemother Date: 2005-02 Impact factor: 5.191

3. Mutations in the central cavity and periplasmic domain affect efflux activity of the resistance-nodulation-division pump EmhB from Pseudomonas fluorescens cLP6a.

Authors: Elizabeth M Hearn; Murray R Gray; Julia M Foght
Journal: J Bacteriol Date: 2006-01 Impact factor: 3.490

Review 4. Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria.

Authors: Laura J V Piddock
Journal: Clin Microbiol Rev Date: 2006-04 Impact factor: 26.132

5. Enhanced expression of the multidrug efflux pumps AcrAB and AcrEF associated with insertion element transposition in Escherichia coli mutants Selected with a fluoroquinolone.

Authors: A S Jellen-Ritter; W V Kern
Journal: Antimicrob Agents Chemother Date: 2001-05 Impact factor: 5.191

6. Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF).

Authors: Jürgen A Bohnert; Sabine Schuster; Eva Fähnrich; Rainer Trittler; Winfried V Kern
Journal: J Antimicrob Chemother Date: 2006-10-24 Impact factor: 5.790

7. Substrate specificity of the RND-type multidrug efflux pumps AcrB and AcrD of Escherichia coli is determined predominantly by two large periplasmic loops.

Authors: Christopher A Elkins; Hiroshi Nikaido
Journal: J Bacteriol Date: 2002-12 Impact factor: 3.490

8. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy.

Authors: O Lomovskaya; M S Warren; A Lee; J Galazzo; R Fronko; M Lee; J Blais; D Cho; S Chamberland; T Renau; R Leger; S Hecker; W Watkins; K Hoshino; H Ishida; V J Lee
Journal: Antimicrob Agents Chemother Date: 2001-01 Impact factor: 5.191

9. Differential impact of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump of Pseudomonas aeruginosa.

Authors: Jocelyn K Middlemiss; Keith Poole
Journal: J Bacteriol Date: 2004-03 Impact factor: 3.490

10. Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors.

Authors: Gaby Sennhauser; Patrick Amstutz; Christophe Briand; Otso Storchenegger; Markus G Grütter
Journal: PLoS Biol Date: 2007-01 Impact factor: 8.029

59 in total

1. Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop.

Authors: Thomas Eicher; Hi-jea Cha; Markus A Seeger; Lorenz Brandstätter; Jasmin El-Delik; Jürgen A Bohnert; Winfried V Kern; François Verrey; Markus G Grütter; Kay Diederichs; Klaas M Pos
Journal: Proc Natl Acad Sci U S A Date: 2012-03-26 Impact factor: 11.205

2. Reversal of the Drug Binding Pocket Defects of the AcrB Multidrug Efflux Pump Protein of Escherichia coli.

Authors: Ketaki Soparkar; Alfred D Kinana; Jon W Weeks; Keith D Morrison; Hiroshi Nikaido; Rajeev Misra
Journal: J Bacteriol Date: 2015-08-03 Impact factor: 3.490

3. The assembled structure of a complete tripartite bacterial multidrug efflux pump.

Authors: Martyn F Symmons; Evert Bokma; Eva Koronakis; Colin Hughes; Vassilis Koronakis
Journal: Proc Natl Acad Sci U S A Date: 2009-04-02 Impact factor: 11.205

4. Site-directed mutagenesis reveals amino acid residues in the Escherichia coli RND efflux pump AcrB that confer macrolide resistance.

Authors: Caroline Wehmeier; Sabine Schuster; Eva Fähnrich; Winfried V Kern; Jürgen A Bohnert
Journal: Antimicrob Agents Chemother Date: 2008-10-20 Impact factor: 5.191