Yasuyuki Mio1, Kazuo Maeda. 1. Reproductive Centre, Mio Fertility Clinic, Tottori University Medical School, Yonago, Japan.
Abstract
OBJECTIVE: The purpose of this study was to clarify developmental changes of early human embryos by using time-lapse cinematography (TLC). STUDY DESIGN: For human ova, fertilization and cleavage, development of the blastocyst, and hatching, as well as consequent changes were repeatedly photographed at intervals of 5-6 days by using an inverse microscope under stabilized temperature and pH. Photographs were taken at 30 frames per second and the movies were studied. RESULTS: Cinematography has increased our understanding of the morphologic mechanisms of fertilization, development, and behavior of early human embryos, and has identified the increased risk of monozygotic twin pregnancy based on prolonged incubation in vitro to the blastocyst stage. CONCLUSION: Using TLC, we observed the fertilization of an ovum by a single spermatozoon, followed by early cleavages, formation of the morula, blastocyst hatching, changes in the embryonic plates, and the development of monozygotic twins from the incubated blastocysts.
OBJECTIVE: The purpose of this study was to clarify developmental changes of early human embryos by using time-lapse cinematography (TLC). STUDY DESIGN: For human ova, fertilization and cleavage, development of the blastocyst, and hatching, as well as consequent changes were repeatedly photographed at intervals of 5-6 days by using an inverse microscope under stabilized temperature and pH. Photographs were taken at 30 frames per second and the movies were studied. RESULTS: Cinematography has increased our understanding of the morphologic mechanisms of fertilization, development, and behavior of early human embryos, and has identified the increased risk of monozygotic twin pregnancy based on prolonged incubation in vitro to the blastocyst stage. CONCLUSION: Using TLC, we observed the fertilization of an ovum by a single spermatozoon, followed by early cleavages, formation of the morula, blastocyst hatching, changes in the embryonic plates, and the development of monozygotic twins from the incubated blastocysts.
Authors: Kirstine Kirkegaard; Johnny Juhl Hindkjaer; Marie Louise Grøndahl; Ulrik Schiøler Kesmodel; Hans Jakob Ingerslev Journal: J Assist Reprod Genet Date: 2012-03-30 Impact factor: 3.412
Authors: A V Karnaukhov; T A Sviridova-Chailakhyan; E V Karnaukhova; M M Panchenko; E V Bogdanenko; L M Chailakhyan Journal: Dokl Biol Sci Date: 2009 May-Jun