Ping Shen1, Yan Jiang, Zhihui Zhou, Junli Zhang, Yunsong Yu, Lanjuan Li. 1. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.
Abstract
OBJECTIVES: The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes. METHODS: The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performed in liquid medium. RESULTS: pKP96 is a circularly closed 67 850 bp multiresistance plasmid with an IncN incompatibility group. Seventy putative genes were identified according to the annotation of the finished sequence. The backbone region of the plasmid, comprising the conjugal transfer and plasmid replication regions, showed 91% identity to the IncN plasmid R46. Several mobile elements were found to be inserted into pKP96 together with antimicrobial resistance genes, including qnrA1, aac(6')-Ib-cr and bla(CTX-M-24). CONCLUSIONS: Plasmid pKP96 is a chimera that has acquired its multiple antimicrobial resistance determinants horizontally from different sources. It may have evolved from an ancestor plasmid similar to R46 through the stepwise events of integration or recombination.
OBJECTIVES: The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes. METHODS: The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performed in liquid medium. RESULTS: pKP96 is a circularly closed 67 850 bp multiresistance plasmid with an IncN incompatibility group. Seventy putative genes were identified according to the annotation of the finished sequence. The backbone region of the plasmid, comprising the conjugal transfer and plasmid replication regions, showed 91% identity to the IncN plasmid R46. Several mobile elements were found to be inserted into pKP96 together with antimicrobial resistance genes, including qnrA1, aac(6')-Ib-cr and bla(CTX-M-24). CONCLUSIONS: Plasmid pKP96 is a chimera that has acquired its multiple antimicrobial resistance determinants horizontally from different sources. It may have evolved from an ancestor plasmid similar to R46 through the stepwise events of integration or recombination.
Authors: V Miriagou; C C Papagiannitsis; S D Kotsakis; A Loli; E Tzelepi; N J Legakis; L S Tzouvelekis Journal: Antimicrob Agents Chemother Date: 2010-07-26 Impact factor: 5.191
Authors: Annemieke Smet; Filip Van Nieuwerburgh; Tom T M Vandekerckhove; An Martel; Dieter Deforce; Patrick Butaye; Freddy Haesebrouck Journal: PLoS One Date: 2010-06-18 Impact factor: 3.240
Authors: Nhu Thi Khanh Nguyen; Vinh Ha; Nga Vu Thieu Tran; Richard Stabler; Duy Thanh Pham; Thi Minh Vien Le; H Rogier van Doorn; Ana Cerdeño-Tárraga; Nicholas Thomson; James Campbell; Van Minh Hoang Nguyen; Thi Thu Nga Tran; Minh Van Pham; Thuy Thu Cao; Brendan Wren; Jeremy Farrar; Stephen Baker Journal: PLoS Negl Trop Dis Date: 2010-06-08