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Literature DB >> 18791223

A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.

Abstract

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

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Year: 2008 PMID： 18791223 PMCID： PMC2581973 DOI： 10.1534/genetics.108.094508

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

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5. A phage integrase directs efficient site-specific integration in human cells.

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