| Literature DB >> 17904350 |
Dagmar Wirth1, Leonor Gama-Norton, Pamela Riemer, Upneet Sandhu, Roland Schucht, Hansjörg Hauser.
Abstract
In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expression properties of a genetically manipulated cell. This review focuses on the current state of targeting strategies including RMCE employing site-specific recombinases such as Cre, Flp and PhiC31. In particular, applications for protein expression, virus production, transgenic animals and chromosome engineering are described.Entities:
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Year: 2007 PMID： 17904350 DOI： 10.1016/j.copbio.2007.07.013
Source DB: PubMed Journal: Curr Opin Biotechnol ISSN： 0958-1669 Impact factor: 9.740