Literature DB >> 18770649

Construction and screening of antigen targeted immune yeast surface display antibody libraries.

Keith D Miller1, Noah B Pefaur, Cheryl L Baird.   

Abstract

These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain antibody fragments (scFvs) is created from antigen-binding B cells from the splenocytes of immunized mice. The antigen targeted library is then screened for antigen specific scFv by magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). Library construction and screening can be accomplished in as little as 2 weeks, resulting in a panel of scFvs specific for the target antigen.

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Year:  2008        PMID： 18770649     DOI： 10.1002/0471142956.cy0407s45

Source DB:  PubMed          Journal:  Curr Protoc Cytom        ISSN： 1934-9297


  8 in total

1.  Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.

Authors:  Keya Zhang; Karan Bhuripanyo; Yiyang Wang; Jun Yin
Journal:  Methods Mol Biol       Date:  2015

Review 2.  Applications of Yeast Surface Display for Protein Engineering.

Authors:  Gerald M Cherf; Jennifer R Cochran
Journal:  Methods Mol Biol       Date:  2015

3.  Engineering the substrate specificity of the DhbE adenylation domain by yeast cell surface display.

Authors:  Keya Zhang; Kathryn M Nelson; Karan Bhuripanyo; Kimberly D Grimes; Bo Zhao; Courtney C Aldrich; Jun Yin
Journal:  Chem Biol       Date:  2013-01-24

4.  Isolation of HCV Neutralizing Antibodies by Yeast Display.

Authors:  Zhen-Yong Keck; Yong Wang; Patrick Lau; Steven K H Foung
Journal:  Methods Mol Biol       Date:  2019

5.  Rare, high-affinity mouse anti-PD-1 antibodies that function in checkpoint blockade, discovered using microfluidics and molecular genomics.

Authors:  Adam S Adler; Rena A Mizrahi; Matthew J Spindler; Matthew S Adams; Michael A Asensio; Robert C Edgar; Jackson Leong; Renee Leong; David S Johnson
Journal:  MAbs       Date:  2017-08-28       Impact factor: 5.857

6.  Prompting Fab Yeast Surface Display Efficiency by ER Retention and Molecular Chaperon Co-expression.

Authors:  Meng Mei; Junhong Li; Shengchen Wang; Ki Baek Lee; Brent L Iverson; Guimin Zhang; Xin Ge; Li Yi
Journal:  Front Bioeng Biotechnol       Date:  2019-11-26

7.  Engineering new protein-protein interactions on the β-propeller fold by yeast cell surface display.

Authors:  Keya Zhang; Heng Li; Karan Bhuripanyo; Bo Zhao; Tiffany F Chen; Ning Zheng; Jun Yin
Journal:  Chembiochem       Date:  2013-02-10       Impact factor: 3.164

8.  Development of smart anti-glycan reagents using immunized lampreys.

Authors:  Tanya R McKitrick; Christoffer K Goth; Charles S Rosenberg; Hirotomo Nakahara; Jamie Heimburg-Molinaro; Alyssa M McQuillan; Rosalia Falco; Nicholas J Rivers; Brantley R Herrin; Max D Cooper; Richard D Cummings
Journal:  Commun Biol       Date:  2020-02-28
  8 in total

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