| Literature DB >> 958484 |
T Alber, G A Petsko, D Tsernoglou.
Abstract
The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.Entities:
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Year: 1976 PMID： 958484 DOI： 10.1038/263297a0
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962