| Literature DB >> 18713617 |
Yee-Guide Yeung1, Edward Nieves, Ruth Hogue Angeletti, E Richard Stanley.
Abstract
Detergents are commonly used for the extraction of hydrophobic proteins and must be removed for sensitive detection of peptides by mass spectrometry. We demonstrate that ethyl acetate is able to extract octylglycoside from a protease digest without loss of peptides or interference with the peptide mass spectral profile. Ethyl acetate extraction was also found to reduce interference by sodium dodecyl sulfate, Nonidet P-40, or Triton X-100 in the mass spectrometry analysis.Entities:
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Year: 2008 PMID： 18713617 PMCID： PMC2584337 DOI： 10.1016/j.ab.2008.07.034
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365