| Literature DB >> 18697906 |
Julie Batut1, Bernhard Schmierer, Jing Cao, Laurel A Raftery, Caroline S Hill, Michael Howell.
Abstract
We identify Balpha (PPP2R2A) and Bdelta (PPP2R2D), two highly related members of the B family of regulatory subunits of the protein phosphatase PP2A, as important modulators of TGF-beta/Activin/Nodal signalling that affect the pathway in opposite ways. Knockdown of Balpha in Xenopus embryos or mammalian tissue culture cells suppresses TGF-beta/Activin/Nodal-dependent responses, whereas knockdown of Bdelta enhances these responses. Moreover, in Drosophila, overexpression of Smad2 rescues a severe wing phenotype caused by overexpression of the single Drosophila PP2A B subunit Twins. We show that, in vertebrates, Balpha enhances TGF-beta/Activin/Nodal signalling by stabilising the basal levels of type I receptor, whereas Bdelta negatively modulates these pathways by restricting receptor activity. Thus, these highly related members of the same subfamily of PP2A regulatory subunits differentially regulate TGF-beta/Activin/Nodal signalling to elicit opposing biological outcomes.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18697906 PMCID： PMC4940033 DOI： 10.1242/dev.020842
Source DB: PubMed Journal: Development ISSN： 0950-1991 Impact factor: 6.868