OBJECTIVE: To study the velocity of sperm DNA fragmentation in frozen-thawed sperm samples from male sperm donors of proved fertility. DESIGN: Sperm DNA fragmentation assessment with use of sperm chromatin dispersion methodology after 0, 4, 8, and 24 hours of incubation in IVF medium. SETTING: Academic biology and reproductive medicine centers. PATIENT(S): Twenty male fertility donors with proved fertility for a maximum of six births at the reproductive medicine center. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The velocity of sperm DNA fragmentation between two consecutive incubations was scored. Best adjustment of sperm DNA fragmentation index versus incubation time for linear, logarithmic, or exponential function was tested. RESULT(S): Increase of sperm DNA fragmentation through time accounted for a substantial percentage of the overall variation. The highest velocity of sperm DNA fragmentation was observed in the first 4 hours of incubation, decreasing by 50% during the second incubation period and being of the order of 1% in the final experimental period. The tendency to increase in sperm DNA fragmentation is not homogeneous among donors; they may adjust to a logarithmic, linear, or exponential function rendering high values for R(2). CONCLUSION(S): Sperm DNA fragmentation occurs rapidly after thawing, and it is an important cause of the rapid decline of sperm quality. Thus, the use of sperm samples as quickly as possible after thawing is highly recommended in clinical practice. Different sperm DNA fragmentation dynamics among individuals were observed.
OBJECTIVE: To study the velocity of sperm DNA fragmentation in frozen-thawed sperm samples from male sperm donors of proved fertility. DESIGN: Sperm DNA fragmentation assessment with use of sperm chromatin dispersion methodology after 0, 4, 8, and 24 hours of incubation in IVF medium. SETTING: Academic biology and reproductive medicine centers. PATIENT(S): Twenty male fertility donors with proved fertility for a maximum of six births at the reproductive medicine center. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The velocity of sperm DNA fragmentation between two consecutive incubations was scored. Best adjustment of sperm DNA fragmentation index versus incubation time for linear, logarithmic, or exponential function was tested. RESULT(S): Increase of sperm DNA fragmentation through time accounted for a substantial percentage of the overall variation. The highest velocity of sperm DNA fragmentation was observed in the first 4 hours of incubation, decreasing by 50% during the second incubation period and being of the order of 1% in the final experimental period. The tendency to increase in sperm DNA fragmentation is not homogeneous among donors; they may adjust to a logarithmic, linear, or exponential function rendering high values for R(2). CONCLUSION(S): Sperm DNA fragmentation occurs rapidly after thawing, and it is an important cause of the rapid decline of sperm quality. Thus, the use of sperm samples as quickly as possible after thawing is highly recommended in clinical practice. Different sperm DNA fragmentation dynamics among individuals were observed.
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