Literature DB >> 18678950

Crystallization and preliminary X-ray analysis of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila.

Shaoqing Yang1, Yaxi Wang, Zhengqiang Jiang, Chengwei Hua.   

Abstract

In this study, the crystallization and preliminary X-ray analysis of a thermostable 1,3-1,4-beta-glucanase produced by Paecilomyces thermophila is described. The purified 1,3-1,4-beta-glucanase was crystallized using the hanging-drop vapour-diffusion method. The crystal belongs to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 154.54, c = 87.62 A. X-ray diffraction data were collected to a resolution of 2.54 A and gave a data set with an overall R(merge) of 7.3% and a completeness of 94.6%. The Matthews coefficient (V(M)) and the solvent content are 2.38 A(3) Da(-1) and 48%, respectively.

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Year:  2008        PMID： 18678950      PMCID： PMC2494974          DOI： 10.1107/S1744309108021064

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  25 in total

1.  Directed mutagenesis of apecific active site residues on Fibrobacter succinogenes 1,3-1,4-beta -D-glucanase significantly affects catalysis and enzyme structural stability.

Authors:  J L Chen; L C Tsai; T N Wen; J B Tang; H S Yuan; L F Shyur
Journal:  J Biol Chem       Date:  2001-03-02       Impact factor: 5.157

2.  Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes.

Authors:  Li-Chu Tsai; Lie-Fen Shyur; Shu-Hua Lee; Su-Shiang Lin; Hanna S Yuan
Journal:  J Mol Biol       Date:  2003-07-11       Impact factor: 5.469

3.  Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin.

Authors:  H Chen; X L Li; L G Ljungdahl
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

4.  A new substrate for investigating the specificity of beta-glucan hydrolases.

Authors:  M A Anderson; B A Stone
Journal:  FEBS Lett       Date:  1975-04-01       Impact factor: 4.124

5.  DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene.

Authors:  R M Teather; J D Erfle
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

Authors:  B Henrissat; A Bairoch
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

8.  Crystal structure of barley 1,3-1,4-beta-glucanase at 2.0-A resolution and comparison with Bacillus 1,3-1,4-beta-glucanase.

Authors:  J J Müller; K K Thomsen; U Heinemann
Journal:  J Biol Chem       Date:  1998-02-06       Impact factor: 5.157

9.  Characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi Trichoderma koningii ZJU-T.

Authors:  J-L Wang; H Ruan; H-F Zhang; Q Zhang; H-B Zhang; G-Q He; S-R Shen
Journal:  J Food Sci       Date:  2007-11       Impact factor: 3.167

10.  Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase.

Authors:  M Hahn; O Olsen; O Politz; R Borriss; U Heinemann
Journal:  J Biol Chem       Date:  1995-02-17       Impact factor: 5.157
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