Literature DB >> 18676700

Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system.

D M Villarreal1, C L Phillips, A M Kelley, S Villarreal, A Villaloboz, P Hernandez, J S Olson, D P Henderson.   

Abstract

To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.

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Year:  2008        PMID： 18676700      PMCID： PMC2547025          DOI： 10.1128/AEM.01291-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Expression of fully functional tetrameric human hemoglobin in Escherichia coli.

Authors:  S J Hoffman; D L Looker; J M Roehrich; P E Cozart; S L Durfee; J L Tedesco; G L Stetler
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.

Authors:  R F Wang; S R Kushner
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

Review 3.  Enhancing stability and expression of recombinant human hemoglobin in E. coli: Progress in the development of a recombinant HBOC source.

Authors:  Philip E Graves; Douglas P Henderson; Molly J Horstman; Brian J Solomon; John S Olson
Journal:  Biochim Biophys Acta       Date:  2008-05-01

4.  High-fidelity translation of recombinant human hemoglobin in Escherichia coli.

Authors:  M J Weickert; I Apostol
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

5.  Characterization of the Plesiomonas shigelloides genes encoding the heme iron utilization system.

Authors:  D P Henderson; E E Wyckoff; C E Rashidi; H Verlei; A L Oldham
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

6.  Turnover of recombinant human hemoglobin in Escherichia coli occurs rapidly for insoluble and slowly for soluble globin.

Authors:  M J Weickert; S R Curry
Journal:  Arch Biochem Biophys       Date:  1997-12-15       Impact factor: 4.013

7.  Iron-binding catechols and virulence in Escherichia coli.

Authors:  H J Rogers
Journal:  Infect Immun       Date:  1973-03       Impact factor: 3.441

8.  A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess.

Authors:  M J Weickert; M Pagratis; C B Glascock; R Blackmore
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792

9.  System for the expression of recombinant hemoproteins in Escherichia coli.

Authors:  Cornelius L Varnado; Douglas C Goodwin
Journal:  Protein Expr Purif       Date:  2004-05       Impact factor: 1.650

10.  Iron uptake in Plesiomonas shigelloides: cloning of the genes for the heme-iron uptake system.

Authors:  P A Daskaleros; J A Stoebner; S M Payne
Journal:  Infect Immun       Date:  1991-08       Impact factor: 3.441
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  10 in total

Review 1.  Development of recombinant hemoglobin-based oxygen carriers.

Authors:  Cornelius L Varnado; Todd L Mollan; Ivan Birukou; Bryan J Z Smith; Douglas P Henderson; John S Olson
Journal:  Antioxid Redox Signal       Date:  2012-11-16       Impact factor: 8.401

2.  Structural basis for hemoglobin capture by Staphylococcus aureus cell-surface protein, IsdH.

Authors:  Kaavya Krishna Kumar; David A Jacques; Gleb Pishchany; Tom Caradoc-Davies; Thomas Spirig; G Reza Malmirchegini; David B Langley; Claire F Dickson; Joel P Mackay; Robert T Clubb; Eric P Skaar; J Mitchell Guss; David A Gell
Journal:  J Biol Chem       Date:  2011-09-14       Impact factor: 5.157

3.  Metal-substituted protein MRI contrast agents engineered for enhanced relaxivity and ligand sensitivity.

Authors:  Victor S Lelyveld; Eric Brustad; Frances H Arnold; Alan Jasanoff
Journal:  J Am Chem Soc       Date:  2011-02-02       Impact factor: 15.419

4.  Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.

Authors:  B J Z Smith; P Gutierrez; E Guerrero; C J Brewer; D P Henderson
Journal:  Appl Environ Microbiol       Date:  2011-07-29       Impact factor: 4.792

5.  Purification of hemoglobin from red blood cells using tangential flow filtration and immobilized metal ion affinity chromatography.

Authors:  Jacob Elmer; David Harris; Andre F Palmer
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2010-12-17       Impact factor: 3.205

Review 6.  Plesiomonas shigelloides Revisited.

Authors:  J Michael Janda; Sharon L Abbott; Christopher J McIver
Journal:  Clin Microbiol Rev       Date:  2016-04       Impact factor: 26.132

7.  Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene.

Authors:  Shuang Meng; Yi Wang; Yan Wang; Changyun Ye
Journal:  Mol Med Rep       Date:  2016-11-14       Impact factor: 2.952

8.  Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene.

Authors:  Shuang Meng; Jianguo Xu; Yanwen Xiong; Changyun Ye
Journal:  PLoS One       Date:  2012-10-15       Impact factor: 3.240

9.  Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli.

Authors:  Paul Quehl; Joel Hollender; Jan Schüürmann; Tatjana Brossette; Ruth Maas; Joachim Jose
Journal:  Microb Cell Fact       Date:  2016-02-02       Impact factor: 5.328

10.  Molecular Basis for the Evolution of Species-Specific Hemoglobin Capture by Staphylococcus aureus.

Authors:  Jacob E Choby; Hanna B Buechi; Allison J Farrand; Eric P Skaar; Matthew F Barber
Journal:  mBio       Date:  2018-11-20       Impact factor: 7.867
  10 in total

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