Literature DB >> 21803893

Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.

B J Z Smith1, P Gutierrez, E Guerrero, C J Brewer, D P Henderson.   

Abstract

We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.

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Year:  2011        PMID： 21803893      PMCID： PMC3187142          DOI： 10.1128/AEM.05712-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

Review 1.  INTERRELATIONSHIP BETWEEN STRUCTURE AND FUNCTION IN HEMOGLOBIN AND MYOGLOBIN.

Authors:  E ANTONINI
Journal:  Physiol Rev       Date:  1965-01       Impact factor: 37.312

2.  Expression of fully functional tetrameric human hemoglobin in Escherichia coli.

Authors:  S J Hoffman; D L Looker; J M Roehrich; P E Cozart; S L Durfee; J L Tedesco; G L Stetler
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

Review 3.  Enhancing stability and expression of recombinant human hemoglobin in E. coli: Progress in the development of a recombinant HBOC source.

Authors:  Philip E Graves; Douglas P Henderson; Molly J Horstman; Brian J Solomon; John S Olson
Journal:  Biochim Biophys Acta       Date:  2008-05-01

4.  High-fidelity translation of recombinant human hemoglobin in Escherichia coli.

Authors:  M J Weickert; I Apostol
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

5.  Simple fed-batch technique for high cell density cultivation of Escherichia coli.

Authors:  D J Korz; U Rinas; K Hellmuth; E A Sanders; W D Deckwer
Journal:  J Biotechnol       Date:  1995-02-21       Impact factor: 3.307

6.  Expression of recombinant human hemoglobin in Escherichia coli.

Authors:  D Looker; A J Mathews; J O Neway; G L Stetler
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

Review 7.  High cell-density culture of Escherichia coli.

Authors:  S Y Lee
Journal:  Trends Biotechnol       Date:  1996-03       Impact factor: 19.536

8.  Stabilization of apoglobin by low temperature increases yield of soluble recombinant hemoglobin in Escherichia coli.

Authors:  M J Weickert; M Pagratis; S R Curry; R Blackmore
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

9.  Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases.

Authors:  U Rinas; K Hellmuth; R Kang; A Seeger; H Schlieker
Journal:  Appl Environ Microbiol       Date:  1995-12       Impact factor: 4.792

10.  A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess.

Authors:  M J Weickert; M Pagratis; C B Glascock; R Blackmore
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792
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  4 in total

Review 1.  Development of recombinant hemoglobin-based oxygen carriers.

Authors:  Cornelius L Varnado; Todd L Mollan; Ivan Birukou; Bryan J Z Smith; Douglas P Henderson; John S Olson
Journal:  Antioxid Redox Signal       Date:  2012-11-16       Impact factor: 8.401

2.  Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers.

Authors:  Michelle Simons; Svetlana Gretton; Gary G A Silkstone; Badri S Rajagopal; Victoria Allen-Baume; Natalie Syrett; Thoufieq Shaik; Nelida Leiva-Eriksson; Luca Ronda; Andrea Mozzarelli; Michael B Strader; Abdu I Alayash; Brandon J Reeder; Chris E Cooper
Journal:  Biosci Rep       Date:  2018-07-02       Impact factor: 3.840

3.  Unnatural biosynthesis by an engineered microorganism with heterologously expressed natural enzymes and an artificial metalloenzyme.

Authors:  Jing Huang; Zhennan Liu; Brandon J Bloomer; Douglas S Clark; Aindrila Mukhopadhyay; Jay D Keasling; John F Hartwig
Journal:  Nat Chem       Date:  2021-10-14       Impact factor: 24.274

4.  Engineering tyrosine residues into hemoglobin enhances heme reduction, decreases oxidative stress and increases vascular retention of a hemoglobin based blood substitute.

Authors:  Chris E Cooper; Gary G A Silkstone; Michelle Simons; Badri Rajagopal; Natalie Syrett; Thoufieq Shaik; Svetlana Gretton; Elizabeth Welbourn; Leif Bülow; Nélida Leiva Eriksson; Luca Ronda; Andrea Mozzarelli; Andras Eke; Domokos Mathe; Brandon J Reeder
Journal:  Free Radic Biol Med       Date:  2018-12-27       Impact factor: 7.376
  4 in total

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