| Literature DB >> 18562038 |
Kazuo Kajita1, Tomoatsu Mune, Takahide Ikeda, Masami Matsumoto, Yoshihiro Uno, Chiyo Sugiyama, Kenji Matsubara, Hiroyuki Morita, Masao Takemura, Mitsuru Seishima, Jun Takeda, Tatsuo Ishizuka.
Abstract
We investigated the effects of fasting on gene expression and intracellular signals regulating energy metabolism in adipose tissue. Following fasting for 15h or 39h, epididymal fat pads were isolated from Wistar rats. PPARgamma mRNA levels decreased in the adipose tissues isolated from rats fasted for 39h, whereas adipocyte lipid-binding protein (aP2) and lipoprotein lipase (LPL) mRNA levels increased. Overnight fasting increased the AMP/ATP ratio and AMP-activated protein kinase (AMPK) in adipose tissue, but not in muscle or liver tissue. In addition, the effect of 5-aminoimidazole-4-carboxyamide-ribonucleoside (AICAR) on PPARgamma expression in primary cultured adipocytes was investigated. AICAR reduced PPARgamma mRNA levels but increased aP2 and LPL mRNA levels. Thus, fasting-induced AMPK activation may affect on the regulation of gene expression in adipocytes.Entities:
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Year: 2008 PMID： 18562038 DOI： 10.1016/j.diabres.2008.05.003
Source DB: PubMed Journal: Diabetes Res Clin Pract ISSN： 0168-8227 Impact factor: 5.602