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Literature DB >> 18542688

Microfluidic CARS cytometry.

Han-Wei Wang¹, Ning Bao, Thuc L Le, Cheng Lu, Ji-Xin Cheng.

Abstract

Coherent anti-stokes Raman scattering (CARS) flow cytometry was demonstrated by combining a laser-scanning CARS microscope with a polydimethylsiloxane (PDMS) based microfluidic device. Line-scanning across the hydrodynamically focused core stream was performed for detection of flowing objects. Parameters were optimized by utilizing polystyrene beads as flowing particles. Population measurements of adipocytes isolated from mouse fat tissues demonstrated the viability of microfluidic CARS cytometry for quantitation of adipocyte size distribution. CARS cytometry could be a new modality for quantitative analysis with vibrational selectivity.

Entities: Chemical Species

Mesh：

Year: 2008 PMID： 18542688 PMCID： PMC2684790 DOI： 10.1364/oe.16.005782

Source DB: PubMed Journal: Opt Express ISSN： 1094-4087 Impact factor: 3.894

15 in total

Review 1. Seventeen-colour flow cytometry: unravelling the immune system.

Authors: Stephen P Perfetto; Pratip K Chattopadhyay; Mario Roederer
Journal: Nat Rev Immunol Date: 2004-08 Impact factor: 53.106

2. Electroporation of mammalian cells in a microfluidic channel with geometric variation.

Authors: Hsiang-Yu Wang; Chang Lu
Journal: Anal Chem Date: 2006-07-15 Impact factor: 6.986

Review 3. Microfluidics for flow cytometric analysis of cells and particles.

Authors: Dongeun Huh; Wei Gu; Yoko Kamotani; James B Grotberg; Shuichi Takayama
Journal: Physiol Meas Date: 2005-02-01 Impact factor: 2.833

4. Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues.

Authors: Yan Fu; Haifeng Wang; Riyi Shi; Ji-Xin Cheng
Journal: Biophys J Date: 2007-02-09 Impact factor: 4.033

5. High-speed, two-photon scanning microscope.

Authors: K H Kim; C Buehler; P T So
Journal: Appl Opt Date: 1999-10-01 Impact factor: 1.980

Review 6. Coherent anti-Stokes Raman Scattering Microscopy.

Authors: Michiel Müller; Andreas Zumbusch
Journal: Chemphyschem Date: 2007-10-22 Impact factor: 3.102

Review 7. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy.

Authors: C D Jennings; K A Foon
Journal: Blood Date: 1997-10-15 Impact factor: 22.113

8. Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues.

Authors: Haifeng Wang; Yan Fu; Phyllis Zickmund; Riyi Shi; Ji-Xin Cheng
Journal: Biophys J Date: 2005-04-15 Impact factor: 4.033

9. Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).

Authors: D C Duffy; J C McDonald; O J Schueller; G M Whitesides
Journal: Anal Chem Date: 1998-12-01 Impact factor: 6.986

10. Single-cell quantification of molecules and rates using open-source microscope-based cytometry.

Authors: Andrew Gordon; Alejandro Colman-Lerner; Tina E Chin; Kirsten R Benjamin; Richard C Yu; Roger Brent
Journal: Nat Methods Date: 2007-01-21 Impact factor: 28.547

14 in total

1. Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.

Authors: Ning Bao; Thuc T Le; Ji-Xin Cheng; Chang Lu
Journal: Integr Biol (Camb) Date: 2010-01-05 Impact factor: 2.192

Microfluidic CARS cytometry.

Review 1. Seventeen-colour flow cytometry: unravelling the immune system.

2. Electroporation of mammalian cells in a microfluidic channel with geometric variation.

Review 3. Microfluidics for flow cytometric analysis of cells and particles.

4. Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues.

5. High-speed, two-photon scanning microscope.

Review 6. Coherent anti-Stokes Raman Scattering Microscopy.

Review 7. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy.

8. Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues.

9. Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).

10. Single-cell quantification of molecules and rates using open-source microscope-based cytometry.

1. Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.

2. Lipid Desaturation Is a Metabolic Marker and Therapeutic Target of Ovarian Cancer Stem Cells.

Review 3. Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy.

4. Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?

Review 5. Recent advances in the use of microfluidic technologies for single cell analysis.

6. Microfluidic tools for cell biological research.

Review 7. Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy.

8. Continuous-flow enzyme assay on a microfluidic chip for monitoring glycerol secretion from cultured adipocytes.

9. Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy.

10. Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis.