Literature DB >> 18324120

High-speed, two-photon scanning microscope.

K H Kim1, C Buehler, P T So.   

Abstract

We have developed a high-speed two-photon microscope with submicrometer resolution in real time. The imaging speed improvement of this system is obtained by the use of a high-speed polygonal mirror scanner. The maximum achievable scanning rate is 40 micros/line, which is approximately 100 times faster than conventional scanning microscopes. High-resolution fluorescence images were recorded in real time by an intensified CCD camera. Using this instrument, we have resolved cellular architecture in three dimensions and have monitored the movements of protozoas. More important, photodamage to biological specimens during video-rate imaging can be minimized with two-photon excitation as compared with other one-photon modalities.

Year:  1999        PMID： 18324120     DOI： 10.1364/ao.38.006004

Source DB:  PubMed          Journal:  Appl Opt        ISSN： 1559-128X            Impact factor:   1.980


  33 in total

1.  Differential Multiphoton Laser Scanning Microscopy.

Authors:  Jeffrey J Field; Kraig E Sheetz; Eric V Chandler; Erich E Hoover; Michael D Young; Shi-You Ding; Anne W Sylvester; David Kleinfeld; Jeff A Squier
Journal:  IEEE J Sel Top Quantum Electron       Date:  2010-10-28       Impact factor: 4.544

2.  Two-photon imaging of spatially extended neuronal network dynamics with high temporal resolution.

Authors:  Kyle P Lillis; Alfred Eng; John A White; Jerome Mertz
Journal:  J Neurosci Methods       Date:  2008-05-03       Impact factor: 2.390

Review 3.  High-throughput nonlinear optical microscopy.

Authors:  Peter T C So; Elijah Y S Yew; Christopher Rowlands
Journal:  Biophys J       Date:  2013-12-17       Impact factor: 4.033

4.  Physical principles for scalable neural recording.

Authors:  Adam H Marblestone; Bradley M Zamft; Yael G Maguire; Mikhail G Shapiro; Thaddeus R Cybulski; Joshua I Glaser; Dario Amodei; P Benjamin Stranges; Reza Kalhor; David A Dalrymple; Dongjin Seo; Elad Alon; Michel M Maharbiz; Jose M Carmena; Jan M Rabaey; Edward S Boyden; George M Church; Konrad P Kording
Journal:  Front Comput Neurosci       Date:  2013-10-21       Impact factor: 2.380

5.  Multifocal multiphoton microscopy based on multianode photomultiplier tubes.

Authors:  Ki Hean Kim; Christof Buehler; Karsten Bahlmann; Timothy Ragan; Wei-Chung A Lee; Elly Nedivi; Erica L Heffer; Sergio Fantini; Peter T C So
Journal:  Opt Express       Date:  2007-09-03       Impact factor: 3.894

Review 6.  Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy.

Authors:  Ramón Carriles; Dawn N Schafer; Kraig E Sheetz; Jeffrey J Field; Richard Cisek; Virginijus Barzda; Anne W Sylvester; Jeffrey A Squier
Journal:  Rev Sci Instrum       Date:  2009-08       Impact factor: 1.523

7.  Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube.

Authors:  Jae Won Cha; Elijah Y S Yew; Daekeun Kim; Jaichandar Subramanian; Elly Nedivi; Peter T C So
Journal:  AIP Adv       Date:  2015-03-23       Impact factor: 1.548

8.  Nonlinear optical microscopy for immunoimaging: a custom optimized system of high-speed, large-area, multicolor imaging.

Authors:  Hui Li; Quan Cui; Zhihong Zhang; Ling Fu; Qingming Luo
Journal:  Quant Imaging Med Surg       Date:  2015-02

9.  Quantification of three-dimensional dynamics of intercellular geometry under mechanical loading using a weighted directional adaptive-threshold method.

Authors:  Nikola Kojic; Austin Huang; Euiheon Chung; Daniel Tschumperlin; Peter T So
Journal:  Opt Express       Date:  2008-08-04       Impact factor: 3.894

10.  SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators.

Authors:  Volodymyr Nikolenko; Brendon O Watson; Roberto Araya; Alan Woodruff; Darcy S Peterka; Rafael Yuste
Journal:  Front Neural Circuits       Date:  2008-12-19       Impact factor: 3.492
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