PURPOSE: To develop elastase-sensitive polyurethane scaffolds that would be applicable to the engineering of mechanically active soft tissues. METHODS: A polyurethane containing an elastase-sensitive peptide sequence was processed into scaffolds by thermally induced phase separation. Processing conditions were manipulated to alter scaffold properties and anisotropy. The scaffold's mechanical properties, degradation, and cytocompatibility using muscle-derived stem cells were characterized. Scaffold in vivo degradation was evaluated by subcutaneous implantation. RESULTS: When heat transfer was multidirectional, scaffolds had randomly oriented pores. Imposition of a heat transfer gradient resulted in oriented pores. Both scaffolds were flexible and relatively strong with mechanical properties dependent upon fabrication conditions such as solvent type, polymer concentration and quenching temperature. Oriented scaffolds exhibited anisotropic mechanical properties with greater tensile strength in the orientation direction. These scaffolds also supported muscle-derived stem cell growth more effectively than random scaffolds. The scaffolds expressed over 40% weight loss after 56 days in elastase containing buffer. Elastase-sensitive scaffolds were complete degraded after 8 weeks subcutaneous implantation in rats, markedly faster than similar polyurethanes that did not contain the peptide sequence. CONCLUSION: The elastase-sensitive polyurethane scaffolds showed promise for application in soft tissue engineering where controlling scaffold mechanical properties and pore architecture are desirable.
PURPOSE: To develop elastase-sensitive polyurethane scaffolds that would be applicable to the engineering of mechanically active soft tissues. METHODS: A polyurethane containing an elastase-sensitive peptide sequence was processed into scaffolds by thermally induced phase separation. Processing conditions were manipulated to alter scaffold properties and anisotropy. The scaffold's mechanical properties, degradation, and cytocompatibility using muscle-derived stem cells were characterized. Scaffold in vivo degradation was evaluated by subcutaneous implantation. RESULTS: When heat transfer was multidirectional, scaffolds had randomly oriented pores. Imposition of a heat transfer gradient resulted in oriented pores. Both scaffolds were flexible and relatively strong with mechanical properties dependent upon fabrication conditions such as solvent type, polymer concentration and quenching temperature. Oriented scaffolds exhibited anisotropic mechanical properties with greater tensile strength in the orientation direction. These scaffolds also supported muscle-derived stem cell growth more effectively than random scaffolds. The scaffolds expressed over 40% weight loss after 56 days in elastase containing buffer. Elastase-sensitive scaffolds were complete degraded after 8 weeks subcutaneous implantation in rats, markedly faster than similar polyurethanes that did not contain the peptide sequence. CONCLUSION: The elastase-sensitive polyurethane scaffolds showed promise for application in soft tissue engineering where controlling scaffold mechanical properties and pore architecture are desirable.
Authors: L K Carr; D Steele; S Steele; D Wagner; R Pruchnic; R Jankowski; J Erickson; J Huard; M B Chancellor Journal: Int Urogynecol J Pelvic Floor Dysfunct Date: 2008-06
Authors: Xinshen Du; Yinping Li; Xing Liu; Xiong Wang; Celine Huselstein; Yanteng Zhao; Peter R Chang; Yun Chen Journal: J Mater Sci Mater Med Date: 2013-12-13 Impact factor: 3.896
Authors: James D Kretlow; Michael C Hacker; Leda Klouda; Brandy B Ma; Antonios G Mikos Journal: Biomacromolecules Date: 2010-03-08 Impact factor: 6.988
Authors: Yi Hong; Sang-Ho Ye; Alejandro Nieponice; Lorenzo Soletti; David A Vorp; William R Wagner Journal: Biomaterials Date: 2009-02-01 Impact factor: 12.479
Authors: Lorenzo Soletti; Yi Hong; Jianjun Guan; John J Stankus; Mohammed S El-Kurdi; William R Wagner; David A Vorp Journal: Acta Biomater Date: 2009-06-18 Impact factor: 8.947