Literature DB >> 18504630

Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20.

Abraham Mathew1, Abi N Eldo, A G Molly.   

Abstract

Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 degrees C and a pH of 5.5 was found to be the optimal pH. The enzyme showed a high level of thermostability in the presence of substrate with a residual activity of 48% after 2 h of incubation at 60 degrees C. A thermostable nature with a high pH range for activity makes it an industrially important enzyme.

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Year:  2008        PMID： 18504630     DOI： 10.1007/s10295-008-0375-0

Source DB:  PubMed          Journal:  J Ind Microbiol Biotechnol        ISSN： 1367-5435            Impact factor:   3.346


  6 in total

Review 1.  Microbial alkaline pectinases and their industrial applications: a review.

Authors:  G S Hoondal; R P Tiwari; R Tewari; N Dahiya; Q K Beg
Journal:  Appl Microbiol Biotechnol       Date:  2002-07-03       Impact factor: 4.813

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Pectinase in papermaking: solving retention problems in mechanical pulps bleached with hydrogen peroxide.

Authors: 
Journal:  Enzyme Microb Technol       Date:  2000-02-01       Impact factor: 3.493

4.  Isolation and characterization of a cellulase-free pectinolytic and hemicellulolytic thermophilic fungus.

Authors:  I Gomes; R K Saha; G Mohiuddin; M M Hoq
Journal:  World J Microbiol Biotechnol       Date:  1992-11       Impact factor: 3.312

5.  Optimization of process for the production of fungal pectinases from deseeded sunflower head in submerged and solid-state conditions.

Authors:  Sarvamangala R Patil; A Dayanand
Journal:  Bioresour Technol       Date:  2005-12-06       Impact factor: 9.642

6.  Production of pectinase from deseeded sunflower head by Aspergillus niger in submerged and solid-state conditions.

Authors:  Sarvamangala R Patil; A Dayanand
Journal:  Bioresour Technol       Date:  2005-11-02       Impact factor: 9.642
  6 in total
  2 in total

1.  Improvement in Thermostability of an Achaetomium sp. Strain Xz8 Endopolygalacturonase via the Optimization of Charge-Charge Interactions.

Authors:  Tao Tu; Huiying Luo; Kun Meng; Yanli Cheng; Rui Ma; Pengjun Shi; Huoqing Huang; Yingguo Bai; Yaru Wang; Lujia Zhang; Bin Yao
Journal:  Appl Environ Microbiol       Date:  2015-07-24       Impact factor: 4.792

2.  Screening and optimization of pectin lyase and polygalacturonase activity from ginseng pathogen Cylindrocarpon Destructans.

Authors:  Gayathri Sathiyaraj; Sathiyaraj Srinivasan; Ho-Bin Kim; Sathiyamoorthy Subramaniyam; Ok Ran Lee; Yeon-Ju Kim; Deok Chun Yang
Journal:  Braz J Microbiol       Date:  2011-06-01       Impact factor: 2.476
  2 in total

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